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Series GSE29263 Query DataSets for GSE29263
Status Public on May 13, 2011
Title Expression data from mouse neonatal ovarian DMBA and BaP culture experiments
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mammalian females are born with a finite number of non-renewing primordial follicles, the majority of which remain in a quiescent state for many years. Due to their non-renewing nature, these “resting” oocytes are particularly vulnerable to xenobiotic insult, resulting in premature ovarian senescence and the formation of dysfunctional oocytes. In this study we characterised the mechanisms of ovotoxicity for the ovotoxic agent 7,12-dimethylbenz-[a]anthracene (DMBA), which targets immature follicles. Neonatal mouse ovaries (PND3-4) were cultured in the presence of DMBA (50nM) or BaP (1uM) for 96 hours to observe their effects on the ovarian transcriptome. This was done in the hopes of gaining a better understanding of the mechanisms underpinning xenobiotic induced pre-antral ovotoxicity.
Overall design Ovaries from day 3-4 Swiss neonatal mice were cultured in DMBA or BaP containing media for 96 hours. The ovaries were then collected for RNA extraction and hybridization on an Illumina Sentrix Mouse ref-8 v2 Beadchip
Contributor(s) Sobinoff AP, McLaughlin EA
Citation(s) 21785161
Submission date May 12, 2011
Last update date Jun 14, 2018
Contact name Alexander Peter Sobinoff
Organization name University of Newcastle Australia
Department Science and IT
Lab Reproductive Science Group
Street address University Drive
City Newcastle
State/province NSW
ZIP/Postal code 2308
Country Australia
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (9)
GSM723302 Control (0.01% Acetone) rep 1
GSM723303 Control (0.01% Acetone) rep 2
GSM723304 Control (0.01% Acetone) rep 3
BioProject PRJNA140269

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29263_RAW.tar 3.1 Mb (http)(custom) TAR
GSE29263_Report-Aceton_vs_BaP.xlsx.gz 3.1 Mb (ftp)(http) XLSX
GSE29263_Report-Aceton_vs_DMBA.xlsx.gz 2.9 Mb (ftp)(http) XLSX
GSE29263_non-normalized.txt.gz 594.6 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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