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| Status |
Public on May 21, 2011 |
| Title |
Expression data from human embryonic stem cells (hESCs) and Activin-induced/derived cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Nodal/Activin signaling directs mesendoderm specification in early vertebrate embryogenesis. We have characterized transcriptional profiling of human embryonic stem cells and Activin-treated cells at different timepoints.
In this dataset, we include the timecourse gene expression data obtained from hESC differentiation post Activin treatment, examining at day 0, 1, 3 and 5.
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| Overall design |
Four biological replicates were used for day 0, three for day 1, three for day 3, and four for day 5. Cells were harvested and used for total RNA preparation using Trizol (Invitrogen). Gene expression was analyzed using GeneChip Human Exon 1.0 ST Array (Affymetrix).
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| Contributor(s) |
Kim SW, Ouyang Z, Wong WH, Baker J |
| Citation(s) |
21741376 |
| Submission date |
May 20, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Si Wan Kim |
| E-mail(s) |
swkimws@gmail.com
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| Organization name |
Stanford University
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| Department |
Genetics
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| Street address |
300 Pasteur Drive M313
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (16)
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| GSM727540 |
hESCs, biological rep4 technical rep1 |
| GSM727541 |
hESCs, biological rep4 technical rep2 |
| GSM727542 |
Activin for day1, biological rep1 |
| GSM727543 |
Activin for day1, biological rep2 |
| GSM727544 |
Activin for day1, biological rep3 |
| GSM727545 |
Activin for day3, biological rep1 |
| GSM727546 |
Activin for day3, biological rep2 |
| GSM727547 |
Activin for day3, biological rep3 |
| GSM727548 |
Activin for day5, biological rep1 |
| GSM727549 |
Activin for day5, biological rep2 |
| GSM727550 |
Activin for day5, biological rep3 |
| GSM727551 |
Activin for day5, biological rep4 technical rep1 |
| GSM727552 |
Activin for day5, biological rep4 technical rep2 |
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| Relations |
| BioProject |
PRJNA141531 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29421_RAW.tar |
346.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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