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Series GSE29444 Query DataSets for GSE29444
Status Public on May 25, 2011
Title Global gene expression of chemical carcinogen (N-methyl-N-nitrosourea), Helicobacter pylori infection, and high-salt diet-treated mouse gastric mucosa
Organism Mus musculus
Experiment type Expression profiling by array
Summary Helicobacter pylori (H. pylori) infection and excessive salt intake are known as important risk factors for stomach cancer in humans. However, interactions of these two factors with gene expression profiles during gastric carcinogenesis remain unclear. In this study, we established a mouse gastric tumor model combining a chemical carcinogen, H. pylori infection and a high-salt diet. The tumor incidence and multiplicity in N-methyl-N-nitrosourea-treated mice were significantly increased by combination of H. pylori with a high-salt diet. In addition, detailed examination indicated that excessive salt could regulate progression of gastric tumor. Global gene expression profiles in glandular stomach of the mouse model were investigated by cDNA microarray analysis, and 36 and 31 more than twofold up-regulated and down-regulated genes, respectively, were detected in the H. pylori-infection and high-salt diet combined group compared with the other groups. Quantitative RT-PCR confirmed significant over-expression of several candidate genes including Cd177, Reg3g, and Muc13. These results suggest that our mouse model combined with H. pylori infection and high-salt diet is useful for gene expression profiling in gastric carcinogenesis.
 
Overall design Five- to six-week-old male C57BL/6J mice (CLEA Japan, Tokyo, Japan) were inoculated with Helicobacter pylori (Sydney strain 1) (Sample No. 2 and 4) or Brucella broth (Sample No. 1 and 3). The animals were administered 120 ppm N-methyl-N-nitrosourea (MNU) in their drinking water on alternate weeks (total exposure, 5 weeks). Mice were given basal diet (CE-2, CLEA Japan) (Sample No. 1 and 2) or high-salt diet containing 10% NaCl (Sample No. 3 and 4). At 40 weeks, the animals were subjected to deep anesthesia and laparotomy with excision of the stomach.
 
Contributor(s) Toyoda T
Citation(s) 23899160
Submission date May 22, 2011
Last update date Aug 04, 2013
Contact name Takeshi Toyoda
Organization name National Institute of Health Sciences
Department Division of Pathology
Street address 1-18-1 Kamiyoga, Setagaya-ku
City Tokyo
ZIP/Postal code 158-8501
Country Japan
 
Platforms (1)
GPL2897 GE Healthcare/Amersham Biosciences CodeLinkā„¢ Mouse Whole Genome Bioarray
Samples (4)
GSM728857 Gastric_mucosa_MNU
GSM728858 Gastric_mucosa_MNU+H. pylori
GSM728859 Gastric_mucosa_MNU+NaCl
Relations
BioProject PRJNA141555

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29444_RAW.tar 3.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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