|
| Status |
Public on May 24, 2011 |
| Title |
Extensive characterization of NF-kappaB binding uncovers non-canonical motifs and advances the interpretation of genetic functional traits |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the DNA binding preferences of 4 NF-kappaB dimers (p52p52, RELARELA, RELAp50, RELAp52)
|
| |
|
| Overall design |
Proteins of the Nf-kappab family were bound to DNA oligonucleotides containing a degenerate region. The protein-DNA complexes were selected after one or multiple rounds of SELEX and the DNA molecules were deep sequenced.
|
| |
|
| Contributor(s) |
Wong D, Teixeira A, Oikonomopoulos S, Humburg P, Lone IN, Saliba D, Siggers T, Bulyk M, Angelov D, Dimitrov S, Udalova I, Ragoussis J |
| Citation(s) |
21801342 |
| Submission date |
May 23, 2011 |
| Last update date |
Dec 03, 2018 |
| Contact name |
Jiannis Ragoussis |
| E-mail(s) |
ioannis.ragoussis@well.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Wellcome Trust Centre for Human Genetics
|
| Street address |
Roosevelt Drive
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7BN |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (6)
|
| GSM729267 |
EMSA-Seq of p52p52 round 1 of SELEX |
| GSM729268 |
EMSA-Seq of p52p52 round 2 of SELEX |
| GSM729269 |
EMSA-Seq of p52p52 round 3 of SELEX |
|
| Relations |
| BioProject |
PRJNA141581 |
Less...
More...