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| Status |
Public on May 24, 2011 |
| Title |
Overexpression of the lung cancer-prognostic miR-146b microRNAs has a minimal and negative effect on the malignant phenotype of A549 lung cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Biological effects of overexpression of miR-146b microRNAs in the A549 human lung cancer cell-line was studied. A549 cells were engineered to express the precursor RNA (pre-miR-146b) that generates the miR-146b microRNAs. Control cells were engineered using the same gene expression plasmid (pLemiR, Open Biosystems®) but without the pre-miR-146b insert. The Trans-Lentiviral GIPZ™ packaging system (Open Biosystems®) was used to generate stable transfectant populations of the engineered cells.
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| Overall design |
Gene expression was compared between A549 stable transfectants of empty pLemiR plasmid (A549/vec cells) or of the pLemiR plasmid containing an insert for overexpressing human pre-miR-146b (A549/146b cells). Two different cell cultures were used for each cell-line (for biological replicates).
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| Contributor(s) |
Patnaik SK, Kannisto E, Mallick R, Yendamuri S |
| Citation(s) |
21789255 |
| Submission date |
May 24, 2011 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Santosh Kumar Patnaik |
| Phone |
716-8458364
|
| Organization name |
Roswell Park Comprehensive Cancer Center
|
| Department |
Thoracic Surgery
|
| Street address |
Elm and Carlton Streets
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
| Samples (4)
|
| GSM729765 |
Empty vector A549 stable transfectants, culture 1 |
| GSM729766 |
Empty vector A549 stable transfectants, culture 2 |
| GSM729767 |
hsa-pre-miR-146b A549 stable transfectants, culture 1 |
| GSM729768 |
hsa-pre-miR-146b A549 stable transfectants, culture 2 |
|
| Relations |
| BioProject |
PRJNA141661 |
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