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Series GSE29616 Query DataSets for GSE29616
Status Public on May 30, 2011
Title Gene expression of primary MMTV-Neu tumors compared to secondary tumors generated from lin- and single tumor initiating cell (TIC) transplantation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Most solid tumors seem to be organized in a hierarchy in which only a fraction of cells, termed tumor-initiating cells (TICs), is capable of disseminating new tumors after transplantation into recipient mice. However, whether a single TIC can induce a tumor or whether it requires additional TICs or non-TICs for tumor initiation is not known. Here we show that injections of single CD24+:Jag1- cells from Her2/Neu+ mammary tumors into recipient mammary glands induced tumors at a frequency of 1/22. Single cell-derived secondary tumors exhibited histology, flow cytometry and global expression profiles that were indistinguishable from primary tumors. Thus, a single TIC can act cell autonomously to induce a tumor and therefore complete eradication of all TICs would be required to cure cancer.
Overall design Total RNA obtained from primary MMTV-Neu tumors, secondary tumors generated by lin- cell transplantation, and secondary tumors generated by a single TIC, were used for microarray analysis to determine differentially regulated genes in the secondary tumors. Cluster analysis based on gene expression were then performed to assess tumor similarity
Contributor(s) Liu J, Deng T, Egan SE, Zacksenhaus E
Citation(s) 22460789
Submission date May 29, 2011
Last update date Jun 14, 2018
Contact name Jeff Liu
Phone 416-340-4800
Organization name University Health Network
Department Cellular and Molecular Biology
Street address 67 College Street, RM427
City Toronto
State/province Ontario
ZIP/Postal code M5G 2M1
Country Canada
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM733926 Primary MMTV-Neu Tumor N86
GSM733927 Primary MMTV-Neu Tumor N133
GSM733928 Primary MMTV-Neu Tumor N135
BioProject PRJNA141361

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29616_RAW.tar 3.1 Mb (http)(custom) TAR
GSE29616_non-normalized.txt.gz 2.5 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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