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| Status |
Public on May 30, 2011 |
| Title |
Gene expression of primary MMTV-Neu tumors compared to secondary tumors generated from lin- and single tumor initiating cell (TIC) transplantation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Most solid tumors seem to be organized in a hierarchy in which only a fraction of cells, termed tumor-initiating cells (TICs), is capable of disseminating new tumors after transplantation into recipient mice. However, whether a single TIC can induce a tumor or whether it requires additional TICs or non-TICs for tumor initiation is not known. Here we show that injections of single CD24+:Jag1- cells from Her2/Neu+ mammary tumors into recipient mammary glands induced tumors at a frequency of 1/22. Single cell-derived secondary tumors exhibited histology, flow cytometry and global expression profiles that were indistinguishable from primary tumors. Thus, a single TIC can act cell autonomously to induce a tumor and therefore complete eradication of all TICs would be required to cure cancer.
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| Overall design |
Total RNA obtained from primary MMTV-Neu tumors, secondary tumors generated by lin- cell transplantation, and secondary tumors generated by a single TIC, were used for microarray analysis to determine differentially regulated genes in the secondary tumors. Cluster analysis based on gene expression were then performed to assess tumor similarity
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| Contributor(s) |
Liu J, Deng T, Egan SE, Zacksenhaus E |
| Citation(s) |
22460789 |
| Submission date |
May 29, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Jeff Liu |
| E-mail(s) |
jeff.liu@utoronto.ca
|
| Phone |
416-340-4800
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| Organization name |
University Health Network
|
| Department |
Princess Margaret Cancer Center
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| Lab |
Zadeh Lab
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| Street address |
101 College Street, RM 4-601
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA141361 |
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