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| Status |
Public on Jun 02, 2011 |
| Title |
The transcriptional repressor Blimp1/Prdm1 regulates post-natal reprogramming of intestinal enterocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Expression profiling of Prdm1 mutant E18.5 small intestine was performed using Illumina whole genome V2 arrays. The hypothesis tested in the present study was that Blimp1 regulates the transcription of key genes involved in enterocyte differentiation and survival. Results identify substantial and premature activation of key components of the adult enterocyte biochemical signature.
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| Overall design |
Villin-Cre and Prdm1BEH/+ animals were intercrossed to generate heterozygous Villin-Cre, Prdm1BEH males that were then mated with homozygous Prdm1 CA/CA females carrying the R26R allele to generate Prdm1+/+ (Prdm1CA/+) or Prdm1-/- (Villin-Cre, Prdm1CA/BEH) offspring. Total RNA obtained from 11 Prdm1+/+ and 10 Prdm1-/- E18.5 small intestine samples was hybridized to Illumina WG6_V2 beadchips.
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| Contributor(s) |
Harper J, Mould A, Andrews R, Bikoff E, Robertson E |
| Citation(s) |
21670299 |
| Submission date |
Jun 01, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Arne Wyndham Mould |
| E-mail(s) |
arne.mould@psych.ox.ac.uk
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| Organization name |
Oxford University
|
| Department |
Psychiatry
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| Lab |
Neural Correlates of Gene Function
|
| Street address |
Warneford Hospital
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 7JX |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA141251 |
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