|
| Status |
Public on May 17, 2012 |
| Title |
Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3' UTRs and Near Stop Codons |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
Here, we use a novel technique for locating regions of N6-adenosine methylation (m6A) throughout the transcriptome and present a profile of m6A sites in the mouse brain. Our use of methylated RNA immunoprecipitation combined with RNA-seq (MeRIP-Seq) identifies thousands of RNAs which contain m6A sites. In addition, we find that regions of m6A formation are particularly enriched near stop codons, which might provide clues into the potential funciton of this highly prevalent RNA modificaiton.
|
| |
|
| Overall design |
Examination of m6A sites in murine brain RNA and human embryonic kidney cells.
|
| |
|
| Contributor(s) |
Meyer KD, Saletore YV, Elemento O, Zumbo P, Mason CE, Jaffrey SR |
| Citation(s) |
22608085 |
| Submission date |
Jun 03, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher E Mason |
| E-mail(s) |
chm2042@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Physiology and Biophysics
|
| Lab |
Christopher Mason Lab
|
| Street address |
1305 York Ave
|
| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (11)
|
| GSM854223 |
C57BL/6 Brain_Sample 1 MeRIP - SYSY |
| GSM854224 |
C57BL/6 Brain_Sample 1 Non-IP Control |
| GSM854225 |
C57BL/6 Brain_Sample 2_Non-IP Control |
|
| Relations |
| BioProject |
PRJNA141085 |
| SRA |
SRP007335 |
Less...
More...