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| Status |
Public on Jun 07, 2011 |
| Title |
Selection of NPC specific Transcription factors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To construct a candidate pool of NPC specific transcription factor (TF), we selected the genes expressed at relatively high levels in NPCs by comparing them to mixed RNA derived from multiple organs, eliminating housekeeping genes. As result, 160 putative NPC specific TFs were selected.
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| Overall design |
We used three independent NPC lines (NSEB5-2C, NS195R, NS21) established from different ES cell lines (EB5, 195R, 21, respectively). Genes exhibiting relatively higher expression (1.5 fold) in NSEB5-2C and NS195R (or, NSEB5-2C and NS21) were selected by comparing with Mixture (consisting of equal microgram of RNAs from heart, kidney, thymus, spleen, liver, lung, d11 embryo). Gene Ontology (GO) terms ‘DNA binding’ and ‘transcription activity’ were used to select TFs.
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| Contributor(s) |
Masui S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 03, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
Takafusa Hikichi |
| E-mail(s) |
thikichi@ri.ncgm.go.jp
|
| Organization name |
National Center for Global Health and Medicine
|
| Street address |
1-21-1 Toyama, Shinjuku-ku
|
| City |
Tokyo |
| ZIP/Postal code |
162-8655 |
| Country |
Japan |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE29875 |
Systematic identification of transcription factors capable of inducing cell-type-specific transcriptional profiles |
|
| Relations |
| BioProject |
PRJNA153873 |
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