|
Status |
Public on Jun 07, 2011 |
Title |
Unsupervised hierarchical clustering of iHPCs induced by 9 or 10 TFs |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
To clarify the gene expression profile of iHep, microarray analysis was performed using iHeps induced by 10 TFs (Foxg1, Lcor, Hnf3b, Hnf4a, Foxo6, Cdx2, Tcf1, Foxa3 ,Tcf2, Onecut1) and 9 TFs (Onecut1 was omitted from 10 TFs). Unsupervised hierarchical clustering indicated that iHep is expressing a global transcriptional profile more similar to that of HPCs rather than that of NPCs, and suggested that TFs present in the pool acted as inducing TFs.
|
|
|
Overall design |
HPC (HB1 and HNG2) were established from fetal liver (E13.5) of C57BL6J and STOCK Tg(Nanog-GFP, Puro)1 Yam, respectively. iHeps were induced from NPC (NSBAg2, established from an ES cell line BAg73C2 carrying beta-geo knock-in allele in Afp) using retroviral vectors (pMXs without drug-selection markers) of 9 or 10 transcription factors. Three weeks after the infection, G418 was added and cultured for 1 week before the harvest. NSBAg2 and NSEB5-2C were used for the data of NPC. GSM396240 and GSM336010 were used for the data of ESC.
|
|
|
Contributor(s) |
Masui S |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Jun 03, 2011 |
Last update date |
May 10, 2018 |
Contact name |
Takafusa Hikichi |
E-mail(s) |
thikichi@ri.ncgm.go.jp
|
Organization name |
National Center for Global Health and Medicine
|
Street address |
1-21-1 Toyama, Shinjuku-ku
|
City |
Tokyo |
ZIP/Postal code |
162-8655 |
Country |
Japan |
|
|
Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
|
Samples (5)
|
|
This SubSeries is part of SuperSeries: |
GSE29875 |
Systematic identification of transcription factors capable of inducing cell-type-specific transcriptional profiles |
|
Relations |
BioProject |
PRJNA153851 |