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Series GSE29730 Query DataSets for GSE29730
Status Public on Jun 07, 2011
Title Unsupervised hierarchical clustering of iHPCs induced by 9 or 10 TFs
Organism Mus musculus
Experiment type Expression profiling by array
Summary To clarify the gene expression profile of iHep, microarray analysis was performed using iHeps induced by 10 TFs (Foxg1, Lcor, Hnf3b, Hnf4a, Foxo6, Cdx2, Tcf1, Foxa3 ,Tcf2, Onecut1) and 9 TFs (Onecut1 was omitted from 10 TFs). Unsupervised hierarchical clustering indicated that iHep is expressing a global transcriptional profile more similar to that of HPCs rather than that of NPCs, and suggested that TFs present in the pool acted as inducing TFs.
 
Overall design HPC (HB1 and HNG2) were established from fetal liver (E13.5) of C57BL6J and STOCK Tg(Nanog-GFP, Puro)1 Yam, respectively. iHeps were induced from NPC (NSBAg2, established from an ES cell line BAg73C2 carrying beta-geo knock-in allele in Afp) using retroviral vectors (pMXs without drug-selection markers) of 9 or 10 transcription factors. Three weeks after the infection, G418 was added and cultured for 1 week before the harvest. NSBAg2 and NSEB5-2C were used for the data of NPC. GSM396240 and GSM336010 were used for the data of ESC.
 
Contributor(s) Masui S
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Submission date Jun 03, 2011
Last update date May 10, 2018
Contact name Takafusa Hikichi
E-mail(s) thikichi@ri.ncgm.go.jp
Organization name National Center for Global Health and Medicine
Street address 1-21-1 Toyama, Shinjuku-ku
City Tokyo
ZIP/Postal code 162-8655
Country Japan
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (5)
GSM737125 HB1
GSM737126 HNG2
GSM737127 iHep-9F
This SubSeries is part of SuperSeries:
GSE29875 Systematic identification of transcription factors capable of inducing cell-type-specific transcriptional profiles
Relations
BioProject PRJNA153851

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29730_RAW.tar 45.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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