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Status |
Public on Aug 19, 2011 |
Title |
HuR (ELAVL1) RIP-chip and knockdown exon array |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array Other
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Summary |
Integrative regulatory mapping indicates that the RNA-binding protein HuR (ELAVL1) couples pre-mRNA processing and mRNA stability In this dataset, we employed two distinct experiments. 1) HuR RIP-chip to identify mRNA targets of HuR. 2) HuR knockdown to identify mRNAs whose expression are dependent on HuR.
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Overall design |
All 12 samples were normalized with PLIER using Affymetrix power tools. To identify RNA targets of HuR, HuR RIP samples were compared to Mock RIP samples. To identify RNA regulated by HuR, HuR knockdown samples were compared to mock knockdown samples.
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Contributor(s) |
Mukherjee N, Keene JD |
Citation(s) |
21723170 |
Submission date |
Jun 07, 2011 |
Last update date |
Feb 18, 2019 |
Contact name |
Neelanjan Mukherjee |
E-mail(s) |
nm29@duke.edu
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Phone |
919-684-1632
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Organization name |
Duke University
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Department |
Institute for Genome Sciences & Policy
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Street address |
101 Science Drive
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City |
Durham |
State/province |
NC |
ZIP/Postal code |
27708 |
Country |
USA |
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Platforms (2) |
GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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Samples (12)
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GSM738173 |
HuR ribonucleoprotein complex immunoprecipitation, biological replicate 1 |
GSM738174 |
HuR ribonucleoprotein complex immunoprecipitation, biological replicate 2 |
GSM738175 |
HuR ribonucleoprotein complex immunoprecipitation, biological replicate 3 |
GSM738176 |
Mock ribonucleoprotein complex immunoprecipitation, biological replicate 1 |
GSM738177 |
Mock ribonucleoprotein complex immunoprecipitation, biological replicate 2 |
GSM738178 |
Mock ribonucleoprotein complex immunoprecipitation, biological replicate 3 |
GSM738179 |
HuR siRNA knockdown, biological replicate 1 |
GSM738180 |
HuR siRNA knockdown, biological replicate 2 |
GSM738181 |
HuR siRNA knockdown, biological replicate 3 |
GSM738182 |
Mock siRNA knockdown, biological replicate 1 |
GSM738183 |
Mock siRNA knockdown, biological replicate 2 |
GSM738184 |
Mock siRNA knockdown, biological replicate 3 |
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This SubSeries is part of SuperSeries: |
GSE29780 |
Integrative regulatory mapping indicates that the RNA-binding protein HuR (ELAVL1) couples pre-mRNA processing and mRNA stability |
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Relations |
BioProject |
PRJNA153883 |