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| Status |
Public on Jun 09, 2011 |
| Title |
Drosophila Staged follicles |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression analysis of yw follicles at S9/10a, S10B, S12, and S14; Gene expression analysis of pxt mutant follicles (f01000 and EY03052) at S10B, S12, S14
Drosophila ovarian follicles complete development using a spatially and temporally controlled maturation process in which they resume meiosis and secrete a multi-layered, protective eggshell before undergoing arrest and/or ovulation. Microarray analysis revealed more than 150 genes that are expressed in a stage-specific manner during the last 24 hours of follicle development. These include all 30 previously known eggshell genes, as well as 19 new candidate chorion genes and 100 other genes likely to participate in maturation. Mutations in pxt, encoding a putative Drosophila cyclooxygenase, cause many transcripts to begin expression prematurely, and are associated with eggshell defects. Somatic activity of Pxt is required, as RNAi knockdown of pxt in the follicle cells recapitulates both the temporal expression and eggshell defects. One of the temporally regulated genes, cyp18a1, which encodes a cytochromome P450 protein mediating ecdysone turnover, is downregulated in pxt mutant follicles, and cyp18a1 mutation itself alters eggshell gene expression. These studies further define the molecular program of Drosophila follicle maturation and support the idea that it is coordinated by lipid and steroid hormonal signals.
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| Overall design |
minimum of 2 replicates per stage and genotype. yw=control follicles. pxt=mutant follicles.
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| Contributor(s) |
Tootle T, Spradling A |
| Citation(s) |
21637834 |
| Submission date |
Jun 08, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Tina Tootle |
| E-mail(s) |
tina-tootle@uiowa.edu
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| URL |
http://www.anatomy.uiowa.edu/personnel.shtml?id=ttootle
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| Organization name |
University of Iowa
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| Department |
Anatomy and Cell Biology
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| Lab |
Tootle Lab
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| Street address |
51 Newton Rd, 1-500BSB
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| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA140933 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29815_RAW.tar |
44.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
| Processed data included within Sample table |
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