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| Status |
Public on Feb 15, 2013 |
| Title |
Long non-coding RNAs regulate adipogenesis (Illumina RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Adipogenesis involves the regulation of hundreds of genes by several well-studied proteins, but the role of long, noncoding RNAs in this process has not been defined. We track the regulation of hundreds of lncRNAs during adipocyte differentiation, and find several that are essential for this process.
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| Overall design |
We extractedbrown and white primary adipocytes and pre-adipocytes and profiled lncRNA expresssion via mRNA-Seq. We also profiled cultured, differentiated adipocytes to verify that we could recapitulate the adipocyte expression profile in preparation for a loss-of-function screen for essential adipogenic lincRNAs.
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| Contributor(s) |
Sun L, Trapnell C, Goff LA, Alexander R, Lo A, Kellis M, Lodish HF, Rinn JL |
| Citation(s) |
23401553 |
| Submission date |
Jun 10, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Cole Trapnell |
| E-mail(s) |
cole@broadinstitute.org
|
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology
|
| Lab |
John Rinn
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (6)
|
| GSM740322 |
RNA-Seq Cultured white pre-adipocytes |
| GSM740323 |
RNA-Seq Cultured white adipocytes |
| GSM740324 |
RNA-Seq Cultured brown pre-adipocytes |
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| This SubSeries is part of SuperSeries: |
| GSE29899 |
Long non-coding RNAs regulate adipogenesis |
|
| Relations |
| SRA |
SRP007112 |
| BioProject |
PRJNA155325 |
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