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| Status |
Public on Jan 06, 2012 |
| Title |
Expression data of pachytene spermatocytes and round spermatids from young and aged Brown Norway rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Male fertility and testis function changes with age and so it was sought to determine if these changes are accompanied by changes in gene expression.
A full genome microarray was used to determine if distinct pathways of genes were altered in expression in germ cells (pachytene spermatocytes or round spermatids) with age.
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| Overall design |
PACH: Testes from young (4 months) and aged (18 months) Brown Norway rats were subjected to a density gradient cell separation to isolate pachytene spermatocytes. RNA was isolated from these cells for hybridization on affymetrix miroarrays.
ROU: Testes from young (4 months) and aged (18 months) Brown Norway rats were subjected to a density gradient cell separation to isolate round spermatids. RNA was isolated from these cells for hybridization on affymetrix miroarrays.
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| Contributor(s) |
Paul C, Robaire B |
| Citation(s) |
21865553, 24391944 |
| Submission date |
Jun 14, 2011 |
| Last update date |
Feb 05, 2020 |
| Contact name |
Bernard Robaire |
| E-mail(s) |
bernard.robaire@mcgill.ca
|
| Organization name |
McGill University
|
| Street address |
3655 Promenade Sir William Osler
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA140839 |
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