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| Status |
Public on Jun 30, 2011 |
| Title |
Comparison of gene expression profiles for hormone induction in the presence and absence of AP1 binding. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression array analysis component. Ligand-dependent transcription by the nuclear receptor glucocorticoid receptor (GR) is mediated by interactions with co-regulators. The role of these interactions in determining selective binding of GR to regulatory elements remains unclear. Recent findings indicate a large fraction of genomic GR binding coincides with chromatin that is accessible prior to hormone treatment, suggesting that receptor binding is dictated by proteins that maintain chromatin in an open state. Combining nucleolytic cleavage and chromatin immunoprecipitation with high-throughput sequencing, we identify the activator protein 1 (AP1) as a major partner for productive GR-chromatin interactions. AP1 is critical for GR-regulated transcription and recruitment to co-occupied regulatory elements, illustrating an extensive AP1-GR interaction network. Importantly, the maintenance of baseline chromatin accessibility facilitates GR recruitment and is dependent on AP1 binding. We propose a model where the basal occupancy of transcription factors act to prime chromatin and direct inducible transcription factors to select regions in the genome.
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| Overall design |
Dexamethasone treatment of cells at zero hour untreated control and 4 hour treatment times, in the absence and presence of acidic-fos (A-fos), an AP1 dominant negative. A-fos expression is under tetracycline control (Tet-Off). This is part of a larger study: Transcription Factor AP1 Potentiates Chromatin Accessibility and Glucocorticoid Receptor Binding. There are 2 replicates per condition per time point.
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| Contributor(s) |
Stamatoyannopoulos JA, Hager GL |
| Citation(s) |
21726817 |
| Submission date |
Jun 15, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
John A Stamatoyannopoulos |
| E-mail(s) |
jstam@altius.org
|
| Organization name |
Altius Institute / University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Stamatoyannopoulos
|
| Street address |
2211 Elliott Avenue, 4th Floor
|
| City |
SEATTLE |
| State/province |
WA |
| ZIP/Postal code |
98121 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
| GSM742082 |
Control 0 hour no tetracycline rep 1 |
| GSM742083 |
Dexamethasone 4 hours no tetracycline rep1 |
| GSM742084 |
Control 0 hour plus tetracycline rep1 |
| GSM742085 |
Dexamethasone 4 hours plus tetracycline rep1 |
| GSM742086 |
Control 0 hour no tetracycline rep2 |
| GSM742087 |
Dexamethasone 4 hours no tetracycline rep2 |
| GSM742088 |
Control 0 hour plus tetracycline rep2 |
| GSM742089 |
Dexamethasone 4 hours plus tetracycline rep2 |
|
| Relations |
| BioProject |
PRJNA140699 |
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