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| Status |
Public on Oct 24, 2011 |
| Title |
The role of HIF in FH-associated renal neoplasia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Fumarate hydratase (FH) mutations predispose to renal cysts cancer. These cancers overexpress hypoxia-inducible factor-alpha (Hif-1a). We have generated a conditional Fh1 (mouse FH) knockout mice that develop renal cysts and overexpress Hif-1a. In order to identify the contribution of Hif-1a to cyst formation we have intercrossed our mice with conditional HIf-1a KO mice.
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| Overall design |
We intercrossed Fh1/Hif1a mice with kidney specific cre recombinase (Ksp-Cre) and analysed kidney cyst formation. RNA was extracted from cysts from 4xFh1 KO, 4xFh1/Hif-1a KO and 4 control mice. For each comparison littermates were used and the animals were aged 15 weeks i.e. early cystic disease.
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| Contributor(s) |
Adam J, Hatipoglu E, O'Flaherty L, Ternette N, Sahgal N, Lockstone H, Baban D, Nye E, Stamp G, Kessler B, Frizzell N, Pugh C, El-Bahrawy M, Ratcliffe P, Pollard P |
| Citation(s) |
22014577 |
| Submission date |
Jun 15, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Natasha Sahgal |
| Organization name |
University of Oxford
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| Department |
Ludwig Institute of Cancer Research
|
| Street address |
Old Road Campus
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| City |
Oxford |
| ZIP/Postal code |
OX3 7DQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA140707 |
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