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Status |
Public on Feb 13, 2012 |
Title |
Analysis of transcript abundance during appressorium formation in the rice blast fungus Magnaporthe oryzae using HT-SuperSAGE. |
Organism |
Pyricularia oryzae |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The rice-blast fungus Magnaporthe oryzae is disseminated using a three-celled spore or conidium. Upon landing on the surface of a rice leaf, the conidium germinates and forms a specialised structure for entry into the host plant - the appressorium. In this study we have followed gene expression thoughout germination of the conidium and formation of the appressorium to identify genes that may be important for appressorium function. We have also compared gene expression in the wild-type germinating conidium at four hours to a mutant strain deleted for the MAP kinase pmk1. The pmk1 mutant is unable to form appressoria.
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Overall design |
Samples were taken from five time-points in wild-type germinating conidia and one time-point in the pmk1 mutant. Two replicates were taken at each time-point.
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Contributor(s) |
Soanes DM, Chakrabarti A, Paszkiewicz KH, Dawe AL, Talbot NJ |
Citation(s) |
22346750 |
Submission date |
Jun 17, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Darren Mark Soanes |
E-mail(s) |
d.m.soanes@exeter.ac.uk
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Organization name |
University of Exeter
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Department |
College of Medicine and Health
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Street address |
RILD Building, RD&E Hospital Wonford, Barrack Road
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City |
Exeter |
ZIP/Postal code |
EX2 5DW |
Country |
United Kingdom |
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Platforms (1) |
GPL13743 |
Illumina Genome Analyzer (Magnaporthe oryzae) |
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Samples (12)
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GSM744258 |
wild-type germinating conidia 4hrs rep1 |
GSM744259 |
wild-type germinating conidia 4hrs rep2 |
GSM744260 |
wild-type germinating conidia 6hrs rep1 |
GSM744261 |
wild-type germinating conidia 6hrs rep2 |
GSM744262 |
wild-type germinating conidia 8hrs rep1 |
GSM744263 |
wild-type germinating conidia 8hrs rep2 |
GSM744264 |
wild-type germinating conidia 14hrs rep1 |
GSM744265 |
wild-type germinating conidia 14hrs rep2 |
GSM744266 |
wild-type germinating conidia 16hrs rep1 |
GSM744267 |
wild-type germinating conidia 16hrs rep2 |
GSM744268 |
pmk1 mutant germinating conidia 4hrs rep1 |
GSM744269 |
pmk1 mutant germinating conidia 4hrs rep2 |
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Relations |
SRA |
SRP007337 |
BioProject |
PRJNA144005 |