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Series GSE30116 Query DataSets for GSE30116
Status Public on Jan 18, 2012
Title Rosiglitazone-induced molecular changes in adipose progenitors
Organism Mus musculus
Experiment type Expression profiling by array
Summary White adipose tissue regulates metabolism; the importance of this control is highlighted by the ongoing pandemic of obesity and associated complications such as diabetes, atherosclerosis, and cancer. White adipose tissue maintenance is a dynamic process, very little is known about how pharmacologic stimuli affect such plasticity. Combining in vivo lineage marking and BrdU labeling strategies, we found that rosiglitazone, a member of the thiazolidinedione class of glucose-lowering medicines, markedly increases the evolution of adipose progenitors into adipocytes. Notably, chronic rosiglitazone administration disrupts the adipogenic and self-renewal capacities of the stem cell compartment and alters its molecular characteristics. These data unravel unknown aspects of adipose dynamics and provide a basis to manipulate the adipose lineage for therapeutic ends.
The goal of this gene expression array was to identify changes in molecular expression in adipose progenitors isolated from mice that underwent two-month rosiglitazone treatment.
 
Overall design Adipose SV GFP+ cells (adipose progenitors) were FACS-isolated from adult AdipoTrak mice that had been treated with or without rosiglitazone (0.0075%) for 2 months. RNAs isolated from these cells were used for microarray. Each cohort contains 3-4 mice, each experimental group (-TZD and +TZD) contains 3 cohorts.
 
Contributor(s) Tang W, Zeve D, Seo J, Jo A, Graff JM
Citation(s) 21723509
Submission date Jun 21, 2011
Last update date Jan 16, 2019
Contact name Wei Tang
E-mail(s) wei.tang@utsouthwestern.edu
Phone 214-648-1482
Fax 214-648-1960
Organization name UT Southwestern Medical Center at Dallas
Department Developmental Biology
Lab Jonathan M. Graff
Street address 6000 Harry Hines Blvd.
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (6)
GSM745628 Adipose No TZD rep1
GSM745629 Adipose TZD rep1
GSM745630 Adipose No TZD rep2
Relations
BioProject PRJNA143869

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30116_RAW.tar 15.8 Mb (http)(custom) TAR
GSE30116_non-normalized.txt.gz 1.9 Mb (ftp)(http) TXT
GSE30116_normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data are available on Series record
Processed data included within Sample table

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