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| Status |
Public on Sep 02, 2013 |
| Title |
Role of Per2 in the control of liver glycogen metabolism |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the role of Per2 in glucose metabolism in-vivo we used mice bearing a targeted gene mutation in the Per2 gene (Per2brdm) and thus unable to express a functional Per2 protein. Mice were hosted in our standard mouse facility in a 12-hour light and 12-hour dark cycle. Wt and Per2brdm mice were fed with either standard chow diet or high-fat diet for 24 weeks and analyzed for glucose homeostasis. Glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed on mice food deprived for 7 hours. For pyruvate tolerance test (PTT) mice were fasted for 14 hours. Glucose levels were measured over 12 hours starvation time-courses during the light and the dark phase. Fed and fasting blood glucose and hepatic glycogen content were measured over different circadian time-points. To investigate the role of Per2 in the control of liver gene expression we performed DNA-microarray analysis of RNA preparations from liver of Per2brdm and WT mice.
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| Overall design |
We extracted total RNA from liver of four different Per2brdm and WT mice. Mice from the same cohort were sacrificed after 8 hours of starvation. After normalization we performed a fold change comparison selecting probes with a p value < 0.05.
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| Contributor(s) |
Zani F, Becattini B, Provenzani A, Stauffer A, Montani J, Albrecht U, Solinas G |
| Citation(s) |
24049741 |
| Submission date |
Jun 22, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Alessandro Provenzani |
| E-mail(s) |
alessandro.provenzani@unitn.it
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| Phone |
+390461283094
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| Organization name |
University of Trento
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| Department |
CIBIO
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| Lab |
Laboratory of Genomic Screening
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| Street address |
via delle regole 101
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| City |
Trento |
| ZIP/Postal code |
38100 |
| Country |
Italy |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA143839 |
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