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| Status |
Public on Jul 30, 2005 |
| Title |
2.45 GHz Radiofrequency fields alter gene expression in cultured human cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by SAGE
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| Summary |
The biological effect of radiofrequency (RF) fields remains controversial. We address this issue by examining whether RF fields can cause changes in gene expression. We observed that 221 genes altered their expression after a 2-hour exposure. The number of affected genes increased to 759 after a 6-hour exposure. Functional classification of the affected genes reveals that apoptosis-related genes were among the up-regulated ones and the cell cycle genes among the down-regulated ones. We observed no significant increase in the expression of heat shock genes. These results indicate that the RF fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism.
Keywords: gene expression SAGE
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| Overall design |
We used the pulsed RF fields at a frequency of 2.45 GHz that is commonly used in telecommunication to expose cultured human HL-60 cells. We used the SAGE (serial analysis of gene expression) method to measure the RF effect on gene expression at the genome level.
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| Contributor(s) |
Lee S, Johnson D, Dunbar K, Dong H, Ge X, Kim YC, Wing C, Jayathilaka N, Emmanuel N, Zhou CQ, Gerber HL, Tseng CC, Wang SM |
| Citation(s) |
16107253 |
| Submission date |
Jul 28, 2005 |
| Last update date |
Apr 18, 2012 |
| Contact name |
San Ming Wang |
| E-mail(s) |
swang1@northwestern.edu
|
| Phone |
224-364-7491
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| Fax |
224-364-5003
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| Organization name |
ENH Research Institute, Northwestern University
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| Department |
Center for Functional Genomics
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| Street address |
1001 University Place
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| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60062 |
| Country |
USA |
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| Platforms (1) |
| GPL4 |
SAGE:10:NlaIII:Homo sapiens |
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| Samples (3) |
| GSM66698 |
Human HL-60 cells |
| GSM66712 |
Human HL-60 cells exposed to 2.45 GHz radiofrequency for 2 hours |
| GSM66714 |
Human HL-60 cells exposed to 2.45 GHz radiofrequency for 6 hours |
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| Relations |
| BioProject |
PRJNA93213 |
