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| Status |
Public on Dec 07, 2012 |
| Title |
Coxiella burnetii infects JEG trophoblastic cell line, a model of placenta infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Q fever is a zoonosis caused by Coxiella burnetii, an obligate intracellular bacterium usually found in myeloid cells. The infection is a source of severe obstetrical complications in humans and cattle, and of chronic evolution in pregnant women. As C. burnetii is found in the placenta of aborted foetuses in humans and ruminants, we wondered if it may infect trophoblasts. In this work, we showed that C. burnetii, infected JEG trophoblastic cells without replication and was localized within phagolysosomes. We analyzed gene expression programs induced by C. burnetii in JEG trophoblastic cell line and compared it with transcriptomic program of BeWo trophoblasts in which C. burnetii replicates. These transcriptomic programs induced by C. burnetii in JEG trophoblasts was poor and markedly different from that induced by C. burnetii in BeWo trophoblasts. Hence, the differences in transcriptomic programs may explain the different intracellular fate of C. burnetii in JEG and BeWo cells. Our results suggest that C. burnetii may use trophoblastic cells as a reservoir by interfering with gene expression.
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| Overall design |
Comparaison between unstimulated JEG cell line and Coxiella burnetii stimulated JEG cell line (bacterial ratio 200:1) for 6 hours
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| Contributor(s) |
Amara AB, Barry AO, Capo C, Ghigo E, Mege J |
| Citation missing |
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| Submission date |
Jun 30, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
adil ef |
| E-mail(s) |
adil.el-filali@univmed.fr
|
| Organization name |
URMITE
|
| Street address |
27 bd jean Moulin
|
| City |
marseille |
| ZIP/Postal code |
13385 cedex 05 |
| Country |
France |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA143619 |
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