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Series GSE30375 Query DataSets for GSE30375
Status Public on Aug 28, 2011
Title Gene expression data from sorted and unsorted primary human acute myeloid leukemia (AML) samples
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Experiments using xenografts show that some solid tumours and leukemias are organized as cellular hierarchies sustained by cancer stem cells (CSC). Despite promise, the relevance of the CSC model to human disease remains uncertain. Here we show that acute myeloid leukemia (AML) follows a CSC model based on sorting multiple populations from each of 16 primary human AML samples and identifying which contain leukemia stem cells (LSC) using a sensitive xenograft assay. Analysis of gene expression from all functionally validated populations yielded an LSC-specific signature. Similarly, a hematopoietic stem cell (HSC) gene signature was established. Bioinformatic analysis identified a core transcriptional program shared by LSC and HSC, revealing the molecular machinery underlying stemness properties. Both stem cell programs were highly significant independent predictors of patient survival and also found in existing prognostic signatures. Thus, determinants of stemness influence clinical outcome of AML establishing that LSC are clinically relevant and not mere artifacts of xenotransplantation.
 
Overall design Analysis of gene expression in FACS sorted AML fractions that were functionally determined to be enriched for LSC or not (25 and 29 respectively).
 
Contributor(s) Eppert K, Dick JE, Ebert BL
Citation(s) 21873988
Submission date Jul 01, 2011
Last update date Jan 17, 2017
Contact name Kolja Eppert
Organization name University Health Network
Street address 101 College St., rm 8-301
City Toronto
State/province Ontario
ZIP/Postal code M5G 1L7
Country Canada
 
Platforms (1)
GPL3921 [HT_HG-U133A] Affymetrix HT Human Genome U133A Array
Samples (93)
GSM753651 1A
GSM753652 1C
GSM753653 1E
This SubSeries is part of SuperSeries:
GSE30377 Human Hematopoietic and Leukemic Stem Cell Gene Expression Profiles
Relations
BioProject PRJNA154731

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30375_RAW.tar 232.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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