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Series GSE30444 Query DataSets for GSE30444
Status Public on Jul 15, 2011
Title Retroviral Sox17 over-expression adult hematopoietic stem/progenitor cells microarray
Organism Mus musculus
Experiment type Expression profiling by array
Summary The transcription factor SOX17 is expressed by fetal, but not adult hematoipoietic stem cells (HSCs), and is required for the maintenance of fetal and neonatal, but not adult, HSCs. In the current study we show that ectopic expression of Sox17 in adult HSCs and transiently reconstituting multipotent progenitors was sufficient to confer increased self-renewal potential and the expression of fetal HSC genes including fetal HSC surface markers.
To assess the mechanisms by which ectopic Sox17 expression in adult hematopoietic progenitors increased self-renewal potential and conferred fetal HSC properties, we compared the gene expression profiles of E16.5 fetal liver HSCs, young adult bone marrow HSCs, young adult bone marrow CD48+LSK cells, and Sox17-expressing CD48+LSK cells isolated from mice that had been transplanted with MSCV-Sox17-infected bone marrow cells 12 weeks earlier.
 
Overall design Total RNA (~5ng) was isolated from 3 independent, freshly isolated aliquots of 10,000 E16.5 fetal liver HSCs, 10,000 fetal liver CD48+LSK cells, 10,000 adult bone marrow HSCs, 10,000 adult bone marrow CD48+LSK cells, 10,000 Sox17-expressing CD48+LSK cells isolated from primary recipients 12 weeks after transplantation of MSCV-Sox17-infected bone marrow cells. Purified RNA was reverse transcribed and amplified using the WT-Ovation™ Pico RNA Amplification system (NuGEN Technologies) following the manufacturer’s instructions. Sense strand cDNA was generated using WT-Ovation™ Exon Module (NuGEN), then fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN). 2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.
 
Contributor(s) He S, Morrison SJ
Citation(s) 21828271
Submission date Jul 06, 2011
Last update date Mar 04, 2019
Contact name Shenghui He
E-mail(s) hesh@umich.edu
Organization name University of Michigan
Department Life Science institute
Lab Sean Morrison
Street address 210 Washtenaw Avenue, 5258 LSI
City Ann Arbor
State/province Michigan
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (12)
GSM755126 E16.5_fetal liver_HSC_Rep1
GSM755127 E16.5_fetal liver_HSC_Rep2
GSM755128 E16.5_fetal liver_HSC_Rep3
This SubSeries is part of SuperSeries:
GSE30446 Transcription factor SOX17 overexpression in hematopoietic stem cells
Relations
BioProject PRJNA154739

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30444_RAW.tar 48.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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