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Status |
Public on Jun 01, 2012 |
Title |
Gene array analysis of embryonic vs adult hypothalamic NPY expressing cell lines |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Microarrays were used to examine gene expression changes between two hypothalamic cell lines derived from adult and embryonic tissues. Both cell lines endogenously express the protein Npy (neuropeptide y) which is involved in feeding and reproductive processes. Results identify novel gene candidates that are implicated in the development of hypothalamic sub-poplulations.
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Overall design |
Immortalized hypothalamic cell lines were created by overexpression of SV40 T-antigen from tissue sources derived from both embryonic and adult mice. Triplicate plates of each cell line were grown to 70% confluence and RNA was harvested for microarray analysis.
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Contributor(s) |
Virtanen C, Dhillon S, Gingerich S, Belsham D |
Citation missing |
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Submission date |
Jul 06, 2011 |
Last update date |
May 04, 2018 |
Contact name |
carl virtanen |
E-mail(s) |
cvirtane@uhnresearch.ca
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Organization name |
University Health Network
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Department |
Princess Margaret Genomics Centre
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Street address |
101 College St
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City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5G 1L7 |
Country |
Canada |
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Platforms (1) |
GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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Samples (6)
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GSM755805 |
Npy expressing, Adult derived A_212 cell line from hypothalamus_1 |
GSM755806 |
Npy expressing, Adult derived A_212 cell line from hypothalamus_2 |
GSM755807 |
Npy expressing, Adult derived A_212 cell line from hypothalamus_3 |
GSM755808 |
Npy expressing, Embryonic derived N_46 cell line from hypothalamus_1 |
GSM755809 |
Npy expressing, Embryonic derived N_46 cell line from hypothalamus_2 |
GSM755810 |
Npy expressing, Embryonic derived N_46 cell line from hypothalamus_3 |
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Relations |
BioProject |
PRJNA143499 |