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| Status |
Public on Jul 31, 2011 |
| Title |
Opposing roles of E2A and Id3 that orchestrate and enforce the naive T cell fate |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
It is established that E2A and its antagonist, Id3, modulate developmental progression at the pre-TCR and TCR checkpoints. Here we show at a global scale how E2A promotes commitment to the T cell lineage and how pre-TCR mediated signalling affects E2A genome-wide occupancy. We find aberrant development of CD4 memory-like and TFH-like cells, T-B cell conjugates and, remarkably, B cell follicles in Id3-/-thymi. We also find that Id3-/-CD4 splenocytes exhibit increased numbers of TFH-like cells. We propose a model in which Id3 modulates the naive versus effector/memory cell fate. Collectively, these data show how E2A acts globally to orchestrate T-lineage development and that Id3 antagonizes E2A activity beyond the pre-TCR checkpoint to enforce the naive T cell fate.
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| Overall design |
ChIP-Seq was performed in thymocytes isolated from either untreated Rag2-/-mice (DN3 cells) or Rag2-/- mice injected with anti-CD3e antibody (DN4 cells). ChIP used antibodies against either E2A or H3K4me1.
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| Contributor(s) |
Miyazaki M, Rivera RR, Miyazaki K, Lin YC, Agata Y, Murre C |
| Citation(s) |
21857655 |
| Submission date |
Jul 08, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Yin Chun Lin |
| E-mail(s) |
yclin@ucsd.edu
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| Organization name |
UCSD
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| Department |
Division of Biological Sciences
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| Lab |
Cornelis Murre
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0377 |
| Country |
USA |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (5)
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| Relations |
| SRA |
SRP007421 |
| BioProject |
PRJNA143293 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30518_RAW.tar |
490.3 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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