|
| Status |
Public on Oct 04, 2005 |
| Title |
Identification of 20E-regulated genes in Drosophila cultured larval organs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify 20E-regulated genes, wandering third instar larvae were dissected and their organs were cultured in the presence of either no hormone, 20E alone, cycloheximide alone, or 20E plus cycloheximide for six hours.
Keywords: Hormone response
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| |
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| Overall design |
Eight partial blue gut third instar larvae were dissected in each well of a 9 well glass dish (Corning) and cultured in ~100 µl oxygenated Schneiders Drosophila Medium (Gibco) at 25°C. Cultures were incubated in an a styrofoam box under a constant flow of oxygen. Following an initial incubation of 1 hour, the medium was removed and replaced with either fresh Schneiders Drosophila Medium (no hormone), medium plus 8.5x10-5 M cycloheximide (Sigma), medium plus 5x10-6 M 20-hydroxyecdysone (Sigma), or medium plus cycloheximide and 20E, each for 6 hrs at 25°C. Organs were collected and RNA extracted from these samples was analyzed on Affymetrix Drosophila Genome Arrays.
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| |
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| Contributor(s) |
Beckstead RB, Lam G, Thummel CS |
| Citation(s) |
16356271 |
| Submission date |
Aug 04, 2005 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Robert B Beckstead |
| E-mail(s) |
robertb@uga.edu
|
| Phone |
706-542-0387
|
| Fax |
706-542-1827
|
| Organization name |
The University of Georgia
|
| Department |
Poultry Science
|
| Lab |
Robert B. Beckstead
|
| Street address |
119 Poultry Science Building
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602-2772 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL72 |
[DrosGenome1] Affymetrix Drosophila Genome Array |
|
| Samples (12)
|
| GSM67156 |
Organ culture non-treated control replicate 1 |
| GSM67157 |
Organ culture non-treated control replicate 2 |
| GSM67158 |
Organ culture non-treated control replicate 3 |
| GSM67159 |
Organ culture 20E-treated replicate 1 |
| GSM67161 |
Organ culture 20E-treated replicate 2 |
| GSM67162 |
Organ culture 20E-treated replicate 3 |
| GSM67165 |
Organ culture 20E+cycloheximide-treated replicate 1 |
| GSM67167 |
Organ culture 20E+cycloheximide-treated replicate 2 |
| GSM67168 |
Organ culture 20E+cycloheximide-treated replicate 3 |
| GSM67170 |
Organ culture cycloheximide-treated replicate 1 |
| GSM67171 |
Organ culture cycloheximide-treated replicate 2 |
| GSM67172 |
Organ culture cycloheximide-treated replicate 3 |
|
| Relations |
| BioProject |
PRJNA93147 |
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