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Series GSE3065 Query DataSets for GSE3065
Status Public on Aug 11, 2005
Title Gene inductions in response to BCR-crosslinking in TAK1-deficient B cell
Organism Mus musculus
Experiment type Expression profiling by array
Summary BCR-induced gene expression profile in wild-type and B cell-specific TAK1-deficient B cells; to elucidate how TAK1 regulates BCR-mediated proliferative response
Keywords: ordered
Overall design Purified splenic B cells (CD43 negative) were treated with or without anti-IgM (20 micloG/mL) for 4 h. Total RNA was extracted with an RNeasy kit (Qiagen), double-stranded DNA was synthesized from 10 micloG of total RNA with the SuperScript Choice System (Invitrogen) primed with T7-(dT) 24 primer. These cDNAs were used to prepare biotin-labeled cRNA by an in vitro transcription reaction performed using T7 RNA polymerase in the presence of biotinylated-ribonucleotides, according to the manufacturer’s protocol (Enzo Diagnostics). The cRNA product was purified using an RNeasy kit, fragmented, and hybridized to Affymetrix mouse expression array A430.2 microarray chips, according to the manufacturer’s protocol (Affymetrix). The hybridized chips were stained, washed, and scanned with a GeneArray Scanner (Affymetrix).
Contributor(s) Sato S
Citation(s) 16186825
Submission date Aug 05, 2005
Last update date Feb 11, 2019
Contact name Shizuo Akira
Organization name Research Institute for Microbial Diseases Osaka University
Department Host Defense
Street address 3-1, Yamada-oka
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM67266 TAK1_WT_unstim
GSM67267 TAK1_WT_IgM
GSM67268 TAK1_KO_unstim
BioProject PRJNA93157

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