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Series GSE30661 Query DataSets for GSE30661
Status Public on Oct 14, 2011
Title SMRT-RID mutation linked to Type I Pneumocyte Associated Respiratory Distress Syndrome via TR repression
Organism Mus musculus
Experiment type Expression profiling by array
Summary We show that knock-in mutations of the nuclear corepressor SMRT in C57Bl6 mice (SMRTmRID) produces a novel respiratory distress syndrome (RDS) due to prematurity of the type I pneumocyte. Treatment with the anti-thyroid hormone drug, propylthiouracil (PTU), completely rescues the SMRT-induced RDS, suggesting an unrecognized and essential role for the thyroid hormone receptor (TR) in lung development. We show that TR and SMRT control type I pneumocyte differentiation through Klf2, which in turn appears to directly activate the type I pneumocyte gene program. Conversely, mice without lung Klf2 lack mature type I pneumocytes and die shortly after birth, closely recapitulating the SMRTmRID phenotype. These results identify a second nuclear receptor, the TR, in type I pneumocyte differentiation and suggest a new type of therapeutic option in the treatment of glucocorticoid non-responsive RDS.
Overall design Total RNA was obtained from WT and SMRT-RID E18.5 lungs of embryos from mothers treated with Diet containing 0.15% PTU or control chow for 2 days (from E16.5).
Contributor(s) Yu RT, Pei L, Evans RM
Citation(s) 22001906
Submission date Jul 14, 2011
Last update date Jun 14, 2018
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM760579 normal wild-type E18.5 lungs, technical replicate 1
GSM760580 normal wild-type E18.5 lungs, technical replicate 2
GSM760581 SMRTmRID E18.5 lungs, technical replicate 1
BioProject PRJNA144589

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30661_RAW.tar 3.1 Mb (http)(custom) TAR
GSE30661_non-normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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