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| Status |
Public on Jul 20, 2011 |
| Title |
High apoptotic threshold mediates p53 dependent decision between arrest and apoptosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In response to stress, the p53 tumor suppressor induces arrest or apoptosis by transcriptionally regulating genes that mediate these processes. It has been proposed that the levels of p53 can influence the choice between these different outcomes, but the mechanisms involved are not clear. To gain mechanistic understanding of this p53-dependent cell fate decision, we generated a p53 inducible system that allowed tight regulation of p53 expression in human mammary epithelial cells.
We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process.
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| Overall design |
Using microarray and chromatin immunoprecipitation analysis, we showed that low and high levels of p53 bind to and activate the same set of pro arrest and pro apoptotic target genes, induced to lower and higher levels, respectively. We propose that the cell fate decision between arrest and apoptosis in these cells is determined by a higher threshold required for p53 dependent apoptosis. We suggest that high level p53 activation is crucial in order to achieve maximum efficacy of p53 targeted cancer therapies.
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| Contributor(s) |
Kracikova M, Akiri G, George A, Sachidanandam R, Lee SA, Aaronson SA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 18, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Ajish D George |
| E-mail(s) |
ajishg@gmail.com
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| Organization name |
Mt Sinai School of Medicine
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| Department |
Dept of Genetics & Genomics
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| Street address |
1425 Madison Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (20)
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| GSM763006 |
time-12h, dosage-3ng, rep-4 |
| GSM763007 |
time-12h, dosage-5ng, rep-1 |
| GSM763008 |
time-12h, dosage-5ng, rep-2 |
| GSM763009 |
time-12h, dosage-5ng, rep-3 |
| GSM763010 |
time-12h, dosage-5ng, rep-4 |
| GSM763011 |
time-24h, dosage-0ng, rep-1 |
| GSM763012 |
time-24h, dosage-0ng, rep-2 |
| GSM763013 |
time-24h, dosage-0ng, rep-3 |
| GSM763014 |
time-24h, dosage-0ng, rep-4 |
| GSM763015 |
time-24h, dosage-3ng, rep-1 |
| GSM763016 |
time-24h, dosage-3ng, rep-2 |
| GSM763017 |
time-24h, dosage-3ng, rep-3 |
| GSM763018 |
time-24h, dosage-3ng, rep-4 |
| GSM763019 |
time-24h, dosage-5ng, rep-1 |
| GSM763020 |
time-24h, dosage-5ng, rep-2 |
| GSM763021 |
time-24h, dosage-5ng, rep-3 |
| GSM763022 |
time-24h, dosage-5ng, rep-4 |
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| Relations |
| BioProject |
PRJNA144127 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30753_RAW.tar |
80.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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