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| Status |
Public on Jul 21, 2011 |
| Title |
Genome-wide mRNA profiling of adult human pancreatic beta and duct cells in comparison to other human tissues |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Aims: establishment of reference samples to investigate gene expression selective for endocrine or ductal-exocrine cells within the adult human pancreas. To this end, human islet endocrine cells, FACS-enriched in insulin+ cells, (n=3) and human exocrine ductal cells (n=2) are compared on Affymetrix HG133A platform with duplicate hybridizations of a panel of other primary human tissues.
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| Overall design |
The microarray analysis was performed on 3 pools of human beta cell-enriched cell fractions, isolated from 10 non-selected donor organs, and 2 pools of duct cell-enriched fractions obtained from 6 non-selected donor pancreases. The cells were suspension-cultured for 2-3 weeks along standard procedures and with no specific treatment prior to FACS-sorting and RNA extraction. The average composition of human beta cell-preparations was 55 ± 13% insulin+ cells, 13±8% glucagon+ cells and 21 ±7% non-granulated cells. Pancreatic duct cells-enriched preparations contained 85 ±7% cytokeratin 19+ cells with 4 ± 1% insulin+ cells and 6 ±4% glucagon+ cells and were isolated as described by Heimberg H. et al.( Diabetes 2001,49: 571-579 ).
Pancreatic cell mRNA profiles were compared to those of a panel of other human primary tissues (n=2 biological replicates).
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| Contributor(s) |
Martens GA, Ling Z, Pipeleers D, Heimberg H |
| Citation(s) |
21912665 |
| Submission date |
Jul 19, 2011 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Geert A. Martens |
| E-mail(s) |
geert.martens@vub.ac.be
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| Organization name |
Vrije Universiteit Brussel
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| Department |
Diabetes Research Center
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| Street address |
Laarbeeklaan 103
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| City |
Brussels |
| ZIP/Postal code |
1090 |
| Country |
Belgium |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (33)
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| GSM764167 |
Human pancreatic beta cell-enriched cell, biological replicate 1 |
| GSM764168 |
Human pancreatic beta cell-enriched cell, biological replicate 2 |
| GSM764169 |
Human pancreatic beta cell-enriched cell, biological replicate 3 |
| GSM764170 |
Human pancreatic duct cells, biological replicate 1 |
| GSM764171 |
Human pancreatic duct cells, biological replicate 2 |
| GSM774041 |
human cerebrum tissue, biological replicate 1 |
| GSM774042 |
human cerebrum tissue, biological replicate 2 |
| GSM774043 |
human cerebellum tissue, biological replicate 1 |
| GSM774044 |
human cerebellum tissue, biological replicate 2 |
| GSM774045 |
human colon tissue, biological replicate 1 |
| GSM774046 |
human colon tissue, biological replicate 2 |
| GSM774047 |
human fetal brain tissue, biological replicate 1 |
| GSM774048 |
human fetal brain tissue, biological replicate 2 |
| GSM774049 |
human kidney tissue, biological replicate 1 |
| GSM774050 |
human kidney tissue, biological replicate 2 |
| GSM774051 |
human liver tissue, biological replicate 1 |
| GSM774052 |
human liver tissue, biological replicate 2 |
| GSM774053 |
human lung tissue, biological replicate 1 |
| GSM774054 |
human lung tissue, biological replicate 2 |
| GSM774055 |
human myocardial tissue, biological replicate 1 |
| GSM774056 |
human skeletal muscle tissue, biological replicate 1 |
| GSM774057 |
human skeletal muscle tissue, biological replicate 2 |
| GSM774058 |
human prostate tissue, biological replicate 1 |
| GSM774059 |
human small intestine tissue, biological replicate 1 |
| GSM774060 |
human small intestine tissue, biological replicate 2 |
| GSM774061 |
human spleen tissue, biological replicate 1 |
| GSM774062 |
human spleen tissue, biological replicate 2 |
| GSM774063 |
human stomach tissue, biological replicate 1 |
| GSM774064 |
human stomach tissue, biological replicate 2 |
| GSM774065 |
human testis tissue, biological replicate 1 |
| GSM774066 |
human testis tissue, biological replicate 2 |
| GSM774067 |
human thymus tissue, biological replicate 1 |
| GSM774068 |
human thymus tissue, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA144453 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30803_RAW.tar |
111.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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