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| Status |
Public on Jul 23, 2011 |
| Title |
Muscle wasting and the temporal gene expresion pattern in a novel rat ICU model |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Acute quadriplegic myopathy (AQM) or critical illness myopathy (CIM) is frequently observed in intensive care unit (ICU) patients. In order to elucidate duration-dependent effects of the ICU intervention on molecular and functional networks that control the muscle wasting and weakness in AQM, gene expression profile was analyzed at time points varying from 6 hours to 14 days in a unique experimental rat model mimicking ICU conditions, i.e., post-synaptically paralyzed, mechanically ventilated and extensively monitored animals.
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| Overall design |
A total of five sham operated controls and 23 experimental female Sprague-Dawley rats were included in this study. The experimental rats were anaesthetized, treated with the neuromuscular blocker (NMBA), α-cobrotoxin, mechanically ventilated and monitored for durations varying from 6h to 4 days (n=13), from 5 to 9 days (n=4), and from 9 to 14 days (n=6). Muscle biopsies were obtained from gastrocnemius muscle (proximal part) immediately after euthanasia and were quickly frozen in liquid propane cooled by liquid nitrogen, and stored at -80°C.RNA was extracted.
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| Contributor(s) |
Llano-Diez M, Gustafson A, Olsson C, Goransson H, Larsson L |
| Citation(s) |
22165895 |
| Submission date |
Jul 21, 2011 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Monica Llano-Diez |
| Organization name |
Uppsala university
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| Department |
Neuroscience
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| Lab |
Clinical neurophysilogy
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| Street address |
Akademiska sjukhuset, Ing.85, 3tr
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| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA144163 |
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