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Series GSE30849 Query DataSets for GSE30849
Status Public on Jul 01, 2012
Title Type I Interferon Dependent Anti-viral Activity
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Synthetic oligonucleotides (ODN) expressing CpG motifs trigger an innate immune response via TLR9. Multiple microarray analyses were performed to identify the global effects on gene expression of stimulating the CAL-1 human pDC line with different types of CpG ODN. Results show that a subset of genes characterized by shared anti-viral activity was consistently up-regulated by ODNs that otherwise mediate discrete functions. This group of genes was largely dependant on autocrine type I interferon (IFN) signaling, as their induction was blocked by neutralizing antibody targeting the type I IFN receptor. Coupling these experiments with a meta-analysis of other published works led to the identification of a set of 32 functionally conserved genes that was reproducibly activated by different types of CpG DNA in different species and cell types. Functionally, these 'core' genes support a type I IFN response to viral infection, and differ from genes up-regulated by only a single type of CpG ODN. These findings help define the conserved and sequence-specific patterns of gene activation triggered via TLR9 and improve our understanding of the immunomodulatory effects elicited by CpG ODN.
Overall design pDC cells were stimulated with either 3uM 'K' ODN for 1-27 hours (1 hr n=3, 3 hr n=3, 9 hr n=5, 27 hr n=3) or with 3uM 'D' ODN for 1-48 hours (1 hr n=3, 3 hr n=3, 9 hr n=3, 27 hr n=5, 48 hr n=3) or with 3uM respective control ODNs: ODN1612 for 9 hours (n=2) and D122 for 27 hours (n=2). Blocking experiments using neutralizing mAb against anti-type I IFNalpha/beta receptor chain2 (MMHAR-2, PBL) (anti-IFNR) were performed: 'K' ODN + anti-IFNR for 9 hours (n=2) and 'D' ODN + anti-IFNR for 27 hours (n=2).
Citation(s) 22750547
Submission date Jul 21, 2011
Last update date Nov 02, 2012
Contact name Folkert Steinhagen
Phone +491788315703
Organization name National Cancer Institute
Street address Breddert 37
City Hilden
ZIP/Postal code 40723
Country Germany
Platforms (1)
GPL3779 NCI/ATC Hs-OperonV3
Samples (45)
GSM765435 'D' ODN for 1 hour - repeat 1 - mAdbID:101300
GSM765436 'K' ODN for 1 hour - repeat 1 - mAdbID:101301
GSM765437 'K' ODN for 3 hours - repeat 1 - mAdbID:101304
BioProject PRJNA144165

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Supplementary file Size Download File type/resource
GSE30849_RAW.tar 171.0 Mb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file
Processed data included within Sample table

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