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| Status |
Public on Jul 15, 2012 |
| Title |
Different effects of 17 beta estrodial and soy protein isolate on bone gene expression in the pre-pubertal female rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Beneficial effects of a soy diet on bone quality have been assumed to be due to the putative estrogenic actions of isoflavones. We studied the effects of soy protein isolate (SPI) on bone quality and compared these effects to 17β-estradiol (E2) in pre-pubertal rats. Female rats were weaned to a control diet with or without E2 (0.1, 1, 10 µg/kg/d), or SPI-containing diet with or without E2 (10 µg/kg/d) for 14 days beginning on postnatal day 20. In long bones from SPI-fed rats, only cancellous bone mineral density (BMD) was increased (p<0.05), while cortical BMD was decreased accompanied by lower bone strength compared to control casein-fed rats (p<0.05). In sharp contrast, 10 µg/kg/d E2 not only increased trabecular BMD, but also cortical BMD compared to controls. Rats treated with the combination of SPI and E2 had an intermediate bone effect. SPI increased while E2 decreased bone turnover, and increased trabecular BMD by both E2 and SPI was associated with decreased serum sclerostin levels. Microarray analysis revealed 652 genes regulated by SPI diet, 491 genes regulated by E2, and 266 genes regulated in common by both SPI diet and E2 compared to rats fed casein. The expression of caveolin-1, a protein localized in cell membrane, was down-regulated (p<0.05) in rats fed SPI, but not by E2 compared to rats fed casein. Down-regulated caveolin-1 by SPI was associated with increased BMP2, Smad and Runx2 expression in bone and osteoblasts (p<0.05). These results suggest SPI consumption results in significant non-classical estrogenic stimulation of cancellous bone formation prior to puberty, but may have adverse effects on overall bone quality and strength at this developmental stage as a result of reduced cortical bone formation.
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| Overall design |
The study was designed to compare expression of bone development associated genes between soy protein isolate fed and estrogen treated pre pubertal female rats. Therefore, total RNA was isolated from bone from rats fed with control casein diet, SPI diet, 17 beta estrodial treated and SPI diet plus 17 beta estrodial.
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| Contributor(s) |
Zhang J, Lazarenko OP, Wu X, Tong Y, Blackburn ML, Badeaux JV, Shankar K, Badger TM, Ronis MJ, Chen J |
| Citation(s) |
22536432 |
| Submission date |
Jul 21, 2011 |
| Last update date |
Sep 23, 2019 |
| Contact name |
Kartik Shankar |
| E-mail(s) |
shankarkartik@uams.edu
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| Phone |
501 364 2847
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| Fax |
501 364 3161
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| Organization name |
University of Arkansas for Medical Sciences/Arkansas Childrens Nutrition Center
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| Department |
Pharmacology and Toxicology
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| Street address |
1120 Marshall Street, Slot 512-20B
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| City |
Little rock |
| State/province |
AR |
| ZIP/Postal code |
72202 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA146151 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30862_RAW.tar |
28.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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