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| Status |
Public on Jul 28, 2011 |
| Title |
Gene expression in hypoxic non-small cell lung cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Hypoxia triggers aggressive cancer growth and contributes to chemotherapy resistance. Novel therapeutic strategies aim at targeting hypoxia activated signaling pathways. Tumor hypoxia not only affects neoplastic tumor cells but also the surrounding stroma cells. Therefore, a novel ex vivo model was established, which allows the study of hypoxia effects in fragments of non-small cell lung cancer (NSCLC) with preserved tumor microenvironment and 3D-structure. Microarray analysis identified 107 significantly regulated genes with at least two-fold expression change in hypoxic compared to normoxic fragments. However, only four genes were significantly regulated in both subtypes, adenocarcinoma and squamous cell carcinoma. The hypoxic regulation of these four genes was verified in an independent set using quantitative PCR.
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| Overall design |
Non-small cell lung cancer (NSCLC) fragments were cultured ex vivo under hypoxia or normoxia for three days. cDNA microarray analysis was performed in hypoxic and normoxic lung cancer fragments from ten patients.
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| |
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| Contributor(s) |
Leithner K, Wagner K, Ebner B, Guelly C |
| Citation(s) |
24460801 |
| Submission date |
Jul 27, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Katharina Leithner |
| E-mail(s) |
katharina.leithner@medunigraz.at
|
| Phone |
+43 316 385 80631
|
| Organization name |
Medical University of Graz
|
| Department |
Department of Internal Medicine, Division of Pulmonology
|
| Street address |
Auenbruggerplatz 20
|
| City |
Graz |
| ZIP/Postal code |
A-8036 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (20)
|
| GSM767776 |
Hypoxic NSCLC fragments patient #1010 |
| GSM767777 |
Hypoxic NSCLC fragments patient #1209 |
| GSM767778 |
Hypoxic NSCLC fragments patient #1210 |
| GSM767779 |
Hypoxic NSCLC fragments patient #1309 |
| GSM767780 |
Hypoxic NSCLC fragments patient #1310 |
| GSM767781 |
Hypoxic NSCLC fragments patient #1409 |
| GSM767782 |
Hypoxic NSCLC fragments patient #1410 |
| GSM767783 |
Hypoxic NSCLC fragments patient #2909 |
| GSM767784 |
Hypoxic NSCLC fragments patient #3209 |
| GSM767785 |
Hypoxic NSCLC fragments patient #3609 |
| GSM767786 |
Normoxic NSCLC fragments patient #1010 |
| GSM767787 |
Normoxic NSCLC fragments patient #1209 |
| GSM767788 |
Normoxic NSCLC fragments patient #1210 |
| GSM767789 |
Normoxic NSCLC fragments patient #1309 |
| GSM767790 |
Normoxic NSCLC fragments patient #1310 |
| GSM767791 |
Normoxic NSCLC fragments patient #1409 |
| GSM767792 |
Normoxic NSCLC fragments patient #1410 |
| GSM767793 |
Normoxic NSCLC fragments patient #2909 |
| GSM767794 |
Normoxic NSCLC fragments patient #3209 |
| GSM767795 |
Normoxic NSCLC fragments patient #3609 |
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| Relations |
| BioProject |
PRJNA146187 |
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