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| Status |
Public on Jun 30, 2012 |
| Title |
Molecular characterisation of circulating tumor cells in human metastatic colorectal cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from the primary tumor into the bloodstream, these Circulating Tumor Cells (CTC) being the principal source of the further metastasis. Here, we approached the massive molecular profiling of the CTC population isolated from mCRC patients. Clinically, the presence of CTC is associated with poor prognosis and there exists a clear necessity for more specific and efficient chemotherapies in the treatment of mCRC. Immunoisolation of CTC from these patients combined with a whole transcriptome amplification and hybridization onto gene expression arrays, lead us to specifically describe for the first time a CTC population with adhesive and migratory characteristics, in addition to a modulated expression of genes related to cell death and survival. Furthermore, bioinformatic analysis provided with an armamentarium of highly specific and sensitive valuable markers that should impact on the management and follow-up of mCRC patients. Finally, molecular profiling of CTC resulted in the identification of new therapeutic targets, like TGF-β1 impairing, specifically targeting the CTC population which should improve the efficacy in the eradication and prevention of CRC metastasis. In conclusion, molecular profiling of CTC represents an innovative and promising approach in the clinical management of mCRC patients.
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| Overall design |
Circulating Tumor Cells (CTC) were immunoisolated from six metastatic colorectal patients (mCRC). Moreover, blood from three healthy donors were obtained, immunoisolating non-specific cellularity background. RNA was extracted and globally amplified with a Whole Transcriptome Amplification system. Samples were hybridized onto agilent arrays and CTC specific genes were obtained. Gene networks were built with IPA software and a gene ontology analysis was performed. Eleven candidate genes were validated by real time PCR. Finally, TGFB1 was evaluated as a potential drugable target against CTC population.
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| Contributor(s) |
Barbazán J, Alonso-Alconada L, Muinelo-Romay L, Vieito M, Abalo A, Alonso-Nocelo M, Candamio S, Gallardo E, Fernández B, Abdulkader I, Casares M, Gómez-Tato A, López-López R, Abal M |
| Citation(s) |
22811761 |
| Submission date |
Jul 28, 2011 |
| Last update date |
Aug 26, 2019 |
| Contact name |
Miguel Abal |
| E-mail(s) |
miguel.abal.posada@sergas.es
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| Phone |
0034981955073
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| Organization name |
University Clinical Hospital
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| Department |
Medical Oncology
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| Lab |
Translational Oncology Laboratory
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| Street address |
Trav Choupana s/n
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| City |
Santiago de Compostela |
| State/province |
A Coruña |
| ZIP/Postal code |
15706 |
| Country |
Spain |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA144915 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31023_RAW.tar |
81.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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