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| Status |
Public on Jul 30, 2011 |
| Title |
The expression profiles of genes using genetically modified parthenogenetic mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Embryonic stem (ES) cells are used in cell therapy and tissue engineering due to their ability to produce different cells types. However, studies of ES cells that are derived from fertilized embryos have raised concerns about the limitations imposed by ethical and political considerations. Therefore, many studies of ES cells use the ES cells that are derived from unfertilized oocytes and adult tissue. Although parthenogenetic embryonic stem (ESP) cells also avoided ethical and political dilemmas and can be used in cell-based therapy, the ESP cells exhibit growth retardation problems. Therefore, to investigate the potential for muscle growth from genetically modified ESP cells, we established four ES cell types, including normal embryonic stem (ESN) cells, ESP cells, ESP cells that overexpress the Igf2 gene (ESI) and ESP cells with down-regulated H19 gene expression (ESH). Using these cells, we examined the expression profiles of genes that were related to imprinting and muscle using microarrays.
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| Overall design |
Total RNA obtained from isolated genetically modified parthenogenetic mouse embryonic stem cells compared to parthenogenetic mouse embryonic stem cells. 2 Biological Replication.
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| Contributor(s) |
Kang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 29, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Jaeku Kang |
| E-mail(s) |
jaeku@konyang.ac.kr
|
| Phone |
82-42-600-6415
|
| Organization name |
College of Medicine, Konyang University
|
| Department |
Department of Pharmacology
|
| Street address |
685, Gasuwon-dong
|
| City |
Seu-gu |
| State/province |
Daejeon |
| ZIP/Postal code |
302-718 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (8)
|
| GSM768906 |
Normal mouse embryonic stem cells_rep1 |
| GSM768907 |
Partenogenetic mouse embryonic stem cells_rep1 |
| GSM768908 |
ESP cells that overexpress Igf2_rep1 |
| GSM768909 |
ESP cells with down-regulated H19 gene expression_rep1 |
| GSM768910 |
Normal mouse embryonic stem cells_rep2 |
| GSM768911 |
Partenogenetic mouse embryonic stem cells_rep2 |
| GSM768912 |
ESP cells that overexpress Igf2_rep2 |
| GSM768913 |
ESP cells with down-regulated H19 gene expression_rep2 |
|
| Relations |
| BioProject |
PRJNA144901 |
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