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Series GSE31031 Query DataSets for GSE31031
Status Public on Jul 30, 2011
Title The expression profiles of genes using genetically modified parthenogenetic mouse embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Embryonic stem (ES) cells are used in cell therapy and tissue engineering due to their ability to produce different cells types. However, studies of ES cells that are derived from fertilized embryos have raised concerns about the limitations imposed by ethical and political considerations. Therefore, many studies of ES cells use the ES cells that are derived from unfertilized oocytes and adult tissue. Although parthenogenetic embryonic stem (ESP) cells also avoided ethical and political dilemmas and can be used in cell-based therapy, the ESP cells exhibit growth retardation problems. Therefore, to investigate the potential for muscle growth from genetically modified ESP cells, we established four ES cell types, including normal embryonic stem (ESN) cells, ESP cells, ESP cells that overexpress the Igf2 gene (ESI) and ESP cells with down-regulated H19 gene expression (ESH). Using these cells, we examined the expression profiles of genes that were related to imprinting and muscle using microarrays.
 
Overall design Total RNA obtained from isolated genetically modified parthenogenetic mouse embryonic stem cells compared to parthenogenetic mouse embryonic stem cells. 2 Biological Replication.
 
Contributor(s) Kang J
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Submission date Jul 29, 2011
Last update date Jun 14, 2018
Contact name Jaeku Kang
E-mail(s) jaeku@konyang.ac.kr
Phone 82-42-600-6415
Organization name College of Medicine, Konyang University
Department Department of Pharmacology
Street address 685, Gasuwon-dong
City Seu-gu
State/province Daejeon
ZIP/Postal code 302-718
Country South Korea
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM768906 Normal mouse embryonic stem cells_rep1
GSM768907 Partenogenetic mouse embryonic stem cells_rep1
GSM768908 ESP cells that overexpress Igf2_rep1
Relations
BioProject PRJNA144901

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31031_RAW.tar 3.1 Mb (http)(custom) TAR
GSE31031_non-normalized.txt.gz 975.4 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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