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| Status |
Public on Nov 05, 2011 |
| Title |
SAGA and ATAC histone acetyl transferase complexes regulate distinct sets of genes and ATAC defines a class of p300-independent enhancers |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Metazoan SAGA and ATAC are distinct multi-subunits complexes that share the same catalytic HAT subunit (GCN5 or PCAF). Here we show that these human HAT complexes are targeted to different genomic loci representing functionally distinct regulatory elements both at broadly expressed and tissue specific genes. While SAGA can principally be found at promoters, ATAC is recruited to promoters and enhancers, yet only its enhancer binding is cell-type specific. Furthermore, we show that ATAC functions at a set of enhancers that are not bound by p300, revealing a new class of enhancers not yet identified. These findings demonstrate important functional differences between SAGA and ATAC coactivator complexes at the level of the genome and define a novel role for the ATAC complex in the regulation of a set of enhancers.
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| Overall design |
Examination of SPT20 in two different cell types.
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| Contributor(s) |
Arnaud K, Karmodiya K, Lindahl-Allen M, Struhl K, Tora L |
| Citation(s) |
22055187 |
| Submission date |
Jul 29, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Krishanpal Karmodiya |
| E-mail(s) |
krish@igbmc.fr, laszlo@igbmc.fr
|
| Organization name |
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)
|
| Street address |
1 rue Laurent Fries / BP 10142
|
| City |
Strasbourg |
| ZIP/Postal code |
67404 |
| Country |
France |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (6)
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| Relations |
| SRA |
SRP007960 |
| BioProject |
PRJNA144857 |
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