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| Status |
Public on Aug 02, 2011 |
| Title |
Expression data from GW8510 treatment of pancreatic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Expression of insulin in terminally differentiated non-beta pancreatic cell types could be important for treating type-1 diabetes. We observed that the kinase inhibitor GW8510 up-regulated insulin expression in mouse pancreatic alpha cells.
Gene-expression profiling and gene-set enrichment analysis of GW8510-treated alpha cells suggested coordinate up-regulation of the p53 pathway, which was further implicated in induction of insulin transcript.
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| Overall design |
In order to determine a potential mechanism of insulin induction by GW8510, we treated mouse alpha cells (aTC1) and beta cells (bTC3) with 3.3 µM GW8510 or 0.1% DMSO for five days, and performed gene-expression profiling followed by gene-set enrichment analysis (GSEA). Three biological replicates were included per condition. GW8510 treatment in each cell line was compared to DMSO-treated control.
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| Contributor(s) |
Fomina-Yadlin D, Wagner B |
| Citation(s) |
22242153 |
| Submission date |
Aug 01, 2011 |
| Last update date |
May 04, 2018 |
| Contact name |
Dina A. Fomina-Yadlin |
| E-mail(s) |
dinafominayadlin@gmail.com
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| Organization name |
Broad Institute
|
| Street address |
7 Cambridge Center, 3112-D
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA144693 |
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