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Series GSE31102 Query DataSets for GSE31102
Status Public on Aug 02, 2011
Title Expression data from GW8510 treatment of pancreatic cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Expression of insulin in terminally differentiated non-beta pancreatic cell types could be important for treating type-1 diabetes. We observed that the kinase inhibitor GW8510 up-regulated insulin expression in mouse pancreatic alpha cells.
Gene-expression profiling and gene-set enrichment analysis of GW8510-treated alpha cells suggested coordinate up-regulation of the p53 pathway, which was further implicated in induction of insulin transcript.
Overall design In order to determine a potential mechanism of insulin induction by GW8510, we treated mouse alpha cells (aTC1) and beta cells (bTC3) with 3.3 ┬ÁM GW8510 or 0.1% DMSO for five days, and performed gene-expression profiling followed by gene-set enrichment analysis (GSEA). Three biological replicates were included per condition. GW8510 treatment in each cell line was compared to DMSO-treated control.
Contributor(s) Fomina-Yadlin D, Wagner B
Citation(s) 22242153
Submission date Aug 01, 2011
Last update date May 04, 2018
Contact name Dina A. Fomina-Yadlin
Organization name Broad Institute
Street address 7 Cambridge Center, 3112-D
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (12)
GSM770002 aTC1_d5_dmso_bio rep1
GSM770003 aTC1_d5_dmso_bio rep2
GSM770004 aTC1_d5_dmso_bio rep3
BioProject PRJNA144693

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31102_RAW.tar 25.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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