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| Status |
Public on Apr 26, 2012 |
| Title |
Impact of FOXA1 over-expression on progestin signalling in transformed normal breast cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genome wide gene expression profiling of response to synthetic progestin ORG2058 in AB32 cells, a PR positive clone of the MCF-10A cell line, was determined after lentiviral transduction with an expression construct
encoding human FOXA1. Cells were treated for 6h or 24h with 10nM ORG2058 or vehicle, 48h after transduction with the FOXA1 construct or empty vector control. Gene expression was measured in total RNA my Illumina whole genome gene expression array.
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| Overall design |
Duplicate monolayer cultures of AB32 cells transduced 48h with pCDH-CMV-FOXA1-EF1-copGFP virus or negative control empty vector pCDH-CMV-mcs-EF1-copGFP virus were treated 6h or 24h with 10nM ORG2058 or 0.1% ethanol vehicle control. Cells were harvested for total RNA isolation and each sample was labelled and hybridized to a single Illumina human whole genome microarray, resulting in three replicate datasets for each of four conditions. Data were analysed separately and combined to give a mean signal intensity for each array probe.
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| Contributor(s) |
Graham JD, Clarke CL |
| Citation(s) |
22545144 |
| Submission date |
Aug 02, 2011 |
| Last update date |
Aug 13, 2018 |
| Contact name |
J Dinny Graham |
| E-mail(s) |
dinny.graham@sydney.edu.au
|
| Phone |
61 2 86273702
|
| Organization name |
University of Sydney
|
| Department |
Westmead Institute for Medical Research
|
| Lab |
Translational Breast Cancer Genomics
|
| Street address |
176 Hawkesbury Rd
|
| City |
Westmead |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE31130 |
Non-overlapping progesterone receptor cistromes contribute to cell-specific transcriptional outcomes in breast cells |
|
| Relations |
| BioProject |
PRJNA154653 |
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