NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE31160 Query DataSets for GSE31160
Status Public on Aug 18, 2011
Title Global gene expression analysis of three different types of mouse motor neurons
Organism Mus musculus
Experiment type Expression profiling by array
Summary Analysis of gene expression differences in three types of mouse motor neurons: (1) those harvested from E13.5 mouse embryos; (2) those derived from embryonic stem cells by directed differentiation; and (3) those made from mouse embryonic fibroblasts by transcription factor reprogramming.
 
Overall design Hb9::GFP+ mouse motor neurons were obtained by three methods [(1) dissection of Hb9::GFP-transgenic E13.5 mouse embryo spinal cord; (2) directed differentiation of an Hb9::GFP-transgenic mouse embryonic stem cell line, V6.5; (3) reprogramming of Hb9::GFP-transgenic mouse embryonic fibroblasts by overexpression of 10 transcription factors]. Each type of motor neuron was purified by FACS and harvested in Trizol. Total RNA was purified and prepared for hybridization onto Illumina MouseRef-8.
 
Contributor(s) Son EY, Ichida JK, Eggan K
Citation(s) 21852222
Submission date Aug 02, 2011
Last update date Jun 14, 2018
Contact name Esther Yesde Son
E-mail(s) son@fas.harvard.edu
Phone 617-496-5611
Fax 617-384-5611
Organization name Harvard University
Department Stem Cells and Regenerative Biology
Lab Eggan
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (10)
GSM771424 Mouse embryonic motor neuron_GFP hi_rep1
GSM771425 Mouse embryonic motor neuron_GFP med_rep1
GSM771426 Mouse embryonic motor neuron_GFP hi_rep2
Relations
BioProject PRJNA144825

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31160_RAW.tar 3.1 Mb (http)(custom) TAR
GSE31160_non-normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap