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Series GSE31185 Query DataSets for GSE31185
Status Public on Nov 01, 2011
Title The human Ewing's Sarcoma oncoprotein EWS-FLI1 causes developmental defects in zebrafish embryos
Organism Danio rerio
Experiment type Expression profiling by array
Summary The fusion oncoprotein EWS-FLI1 arises from a t(11;22)(q24;q12) chromosomal translocation and causes Ewing's Sarcoma, a malignant bone tumor. The mechanism whereby EWS-FLI1 transforms cells is unknown. We made germline transgenic zebrafish expressing human EWS-FLI1 under the control of the heat shock promoter. Induction of EWS-FLI1 expression causes multiple defects in embryonic development.
We compared gene expression in control and transgenic EWS-FLI1 zebrafish. The results identify a conserved set of EWS-FLI1-regulated genes, and provide insight into the pathogenesis of Ewing's Sarcoma tumors.
Overall design We performed heat shock and isolated total RNA for microarray studies comparing wildtype AB strain zebrafish with transgenic zebrafish expressing human EWS-FLI1 [Tg(HSP:EWS-FLI1)]. RNA was biotin-lableled and hybridized to zebrafish-specific Affymetrix arrays.
Contributor(s) Leacock S, Amatruda JF
Citation(s) 21979944
Submission date Aug 03, 2011
Last update date Jan 25, 2018
Contact name James F Amatruda
Phone 214-648-3896
Organization name University of Texas Southwestern Medical Center
Department Pediatrics and Molecular Biology
Street address 5323 Harry Hines Boulevard
City Dallas
State/province TX
ZIP/Postal code 75390-8534
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM773079 AB control embryos biological rep1
GSM773080 AB control embryos biological rep2
GSM773081 AB control embryos biological rep3
This SubSeries is part of SuperSeries:
GSE31217 A zebrafish transgenic model of Ewing's Sarcoma reveals conserved mediators of EWS-FLI1 tumorigenesis
BioProject PRJNA154077

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31185_RAW.tar 11.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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