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| Status |
Public on Sep 13, 2012 |
| Title |
Methylation map of 8 neuroblastoma cell lines: NGS after MBD2-capture |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
8 neuroblastoma (NB) cell lines (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) their methylome is determined by sequencing after MBD2-capture using MethylCollector (ActiveMotif)
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| Overall design |
8 NB cell lines were included (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) in this study. After shearing (fragments of about 200 bp), DNA was captured using MBD2-capture (MethylCollector - ActiveMotif) followed by library preparation and multiplexing. Captured sequence tags were sequenced paired-end (2 x 45 bp) on Illumina GAIIx.
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| Contributor(s) |
Decock A, Ongenaert M, Vandesompele J, Speleman F |
| Citation(s) |
23034519 |
| Submission date |
Aug 12, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Maté Ongenaert |
| E-mail(s) |
mate.ongenaert@gmail.com
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| Organization name |
Ghent University
|
| Department |
Center for Medical Genetics
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| Lab |
Lab. Cytometry
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| Street address |
De Pintelaan 185 - MRB
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| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE31355 |
A genome wide methylation map of neuroblastoma cell lines |
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| Relations |
| SRA |
SRP007898 |
| BioProject |
PRJNA154163 |
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