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| Status |
Public on Aug 19, 2014 |
| Title |
miRNA Expression data from WASF3 overexpressing stable T47D cells and control cells |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
We recently showed that inactivation of the WASF3/WAVE3 gene in breast cancer cells results in loss of cell motility and invasion in vitro and metastasis in vivo. To obtain a better understanding of molecular mechanisms of action of WASF3, we have established the stable WASF3 overexpressing T47D cells using lentiviral infect system.
We used miRNA microarrays to detail the global programme of miRNA expression after overexpression of WASF3 and identified distinct classes of up or down-regulated metastmir associated with breast cancer cell migration and motility invasion
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| Overall design |
total RNA including small RNA was extracted from WASF3 overexpressing and empty vector transfected T47D cells, then labeled RNAs were hybridized on GeneChip miRNA Array. To identify altered miRNA expression patterns before or after overexpression of WASF3, we compared average miRNA expression levels between WASF3 overexpressing and control T47D cells.
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| Contributor(s) |
Teng Y, Cowell JK |
| Citation missing |
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| Submission date |
Aug 12, 2011 |
| Last update date |
May 02, 2017 |
| Contact name |
yong teng |
| E-mail(s) |
gzyteng@yahoo.com.cn
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| Organization name |
Medical College of Georgia
|
| Street address |
1120 15th Street
|
| City |
Augusta |
| ZIP/Postal code |
30912 |
| Country |
USA |
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| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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| Samples (4)
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| GSM777675 |
Empty vector transfected T47D-rep 2 |
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| Relations |
| BioProject |
PRJNA145889 |
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