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Series GSE31390 Query DataSets for GSE31390
Status Public on Dec 30, 2011
Title Gene expression profile of Tra1 dependent genes
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Promoter-specific transcriptional activators (activators) stimulate transcription through direct interactions with one or more components of the transcription machinery, termed the “target”. Previous studies have provided evidence that the Tra1 subunit of the yeast SAGA (Spt-Ada-Gcn5-acetyltransferase) complex is the target of the yeast activator Gal4. However, several other general transcription factors, in particular the mediator complex, have also been implicated as Gal4 targets. To investigate the essentiality of Tra1 as a target of Gal4, here we derive Tra1 mutants that are selectively defective for interaction with Gal4 in vivo (Gal4 Interaction Defective (GID) mutants). In contrast to wild-type Tra1, Tra1 GID mutants are not recruited by Gal4 to the promoter and cannot support Gal4-directed transcription activation, demonstrating that the Gal4–Tra1 interaction is required for Gal4 function. In yeast strains expressing a Tra1 GID mutant, Gal4 promoter binding is unexpectedly also diminished indicating that Gal4 and Tra1 bind cooperatively. Consistent with cooperative binding, we demonstrate that the interaction between Gal4 and Tra1 occurs predominantly on the promoter and not off DNA. Finally, we show that although Tra1 is also targeted by other activators, these interaction are unaffected by GID mutations, revealing an unanticipated specificity of the Gal4-Tra1 interaction.
 
Overall design Strain MDC1(TRA1 WT) and MDC3 (Tra1 ts) were grown at 30˚C and then shifted to 37˚C for 60, 90 and 120 mins.
 
Contributor(s) Lin L
Citation(s) 22308403
Submission date Aug 15, 2011
Last update date Jul 01, 2016
Contact name Michael R. Green
E-mail(s) michael.green@umassmed.edu
Organization name University of Massachusetts Medical School
Department Molecular, Cell and Cancer Biology
Lab Michael R. Green
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (6)
GSM778739 WT1
GSM778740 WT2
GSM778741 WT3
This SubSeries is part of SuperSeries:
GSE31391 Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4
Relations
BioProject PRJNA154143

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31390_RAW.tar 7.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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