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Series GSE31413 Query DataSets for GSE31413
Status Public on Aug 11, 2014
Title Nanog-induced intestinal and colonic hyperplasia
Organism Mus musculus
Experiment type Expression profiling by array
Summary Though expression of the homeobox transcription factor Nanog is generally restricted to pluripotent cells and early germ cells, recent reports have found that Nanog is re-expressed in somatic and germ cell tumors associated with poor differentiation and aggressive disease progression.
To elucidate its oncogenic properties, a modified Tet-On system was utilised to generate Nanog-inducible mice.
By dexamethasone and doxycycline injection, similar Nanog expression levels as in wild-type embryonic stem cells were obtained in all major organs except for brain.
Ectopic Nanog expression resulted in intestinal and colonic epithelium hyperplasia-intestinal villi had doubled in length and hyperplastic epithelium outgrowths were seen after 7 days.
The tumor suppressor gene Cdx2 was downregulated at onset of Nanog expression, suggesting that crosstalk between Nanog and Cdx2 is conserved from early embryo development to adulthood.
Although the mice died before they could form tumors, Cdx2 repression and intestinal hyperplasia links Nanog to tumor initiation.
 
Overall design 8 samples were analyzed.
Intestine-: Mouse intestine cells without Nanog overexpression, 2 biological rep
Intestine+: Mouse intestine cells with Nanog overexpression, 2 biological rep
Colon-: Mouse colon cells without Nanog overexpression, 2 biological rep
Colon+: Mouse colon cells with Nanog overexpression, 2 biological rep
 
Contributor(s) Fishedick G, Wu G, Greber B, Araúzo-Bravo MJ, Köhler G, Tapia N, Iacone R, Anastassiadis K, Zaehres H, Schöler HR
Citation(s) 25173648
Submission date Aug 16, 2011
Last update date Jun 14, 2018
Contact name Marcos J Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM779388 Intestine- rep1
GSM779389 Intestine- rep2
GSM779390 Intestine+ rep1
Relations
BioProject PRJNA145745

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31413_Non-Normalized.txt.gz 1.4 Mb (ftp)(http) TXT
GSE31413_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data are available on Series record
Processed data included within Sample table

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