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Series GSE31414 Query DataSets for GSE31414
Status Public on May 03, 2012
Title ART culture conditions change the probability of mouse embryo gestation through defined cellular and molecular responses
Organism Mus musculus
Experiment type Expression profiling by array
Summary With the increasing use of Assisted Reproductive Technologies (ART) for treatment of human infertility, there is an increasing requirement for embryo culture conditions that perform as similar to nature as possible.
How good the match, however, cannot be tested experimentally in human.
We solved the central question of how well ART culture protocols prepare embryos for postimplantation development, under the provisions of the 'mouse embryo assay' (MEA).
Our side-by-side comparison of 8 conditions [i.e., 3 culture conditions (KSOM, HTF and ISDM1) plus the in vivo system in two different mouse strains (B6 and CD1)] shows that mouse embryos cultured under ART conditions are differentially primed for postimplantation development, and that certain ART protocols outperform the oviduct.
The distinct performances of blastocysts formed in ART vs. oviduct do not correlate with any significant transcriptome changes, whereas protein analysis by immunoconfocal microscopy reveals differences in the allocation of embryonic cells to the three germ layers of blastocysts.
We conclude that in vitro technology is not always a defective copy of nature, and that the choice of ART protocol primes the embryos for subsequent development.
 
Overall design 22 samples were analyzed.
B6KSOM: Mouse B6 background, E3.5 blastocysts in KSOM medium, 3 biological rep
B6HTF: Mouse B6 background, E3.5 blastocysts in HTF medium, 3 biological rep
B6ISM1: Mouse B6 background, E3.5 blastocysts in ISM1 medium, 3 biological rep
B6vivo: Mouse B6 background, E3.5 blastocysts in vivo, 3 biological rep
CD1KSOM: Mouse CD1 background, E3.5 blastocysts in KSOM medium, 1 biological rep
CD1HTF: Mouse CD1 background, E3.5 blastocysts in HTF medium, 3 biological rep
CD1ISM1: Mouse CD1 background, E3.5 blastocysts in ISM1 medium, 3 biological rep
CD1vivo: Mouse CD1 background, E3.5 blastocysts in vivo, 3 biological rep
 
Contributor(s) Araúzo-Bravo MJ, Boiani M, Esteves TC, Le Gac S, Nordhoff V, Schlatt S, Schwarzer C
Citation(s) 22736328
Submission date Aug 16, 2011
Last update date Jun 14, 2018
Contact name Marcos J Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (22)
GSM779396 B6KSOM rep1
GSM779397 B6KSOM rep2
GSM779398 B6KSOM rep3
Relations
BioProject PRJNA145747

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31414_Non-Normalized.txt.gz 3.7 Mb (ftp)(http) TXT
GSE31414_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data are available on Series record
Processed data included within Sample table

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