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| Status |
Public on Aug 19, 2011 |
| Title |
Exposure to Nickel, Chromium, or Cadmium Causes Distinct Changes in the Gene Expression Patterns of a Rat Liver Derived Cell Line |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Many heavy metals, including nickel (Ni), cadmium (Cd), and chromium (Cr) are toxic industrial chemicals with an exposure risk in both occupational and environmental settings that may cause harmful outcomes. While these substances are known to produce adverse health effects leading to disease or health problems, the detailed mechanisms remain unclear. To elucidate the processes involved in the of toxicity of nickel, cadmium, and chromium at the molecular level and to perform a comparative analysis, H4-II-E-C3 rat liver-derived cell lines were treated with soluble salts of each metal using concentrations derived from viability assays, and gene expression patterns were determined with DNA microarrays.
We identified both common and unique biological responses to exposure to the three metals. Nickel, cadmium, chromium all induced oxidative stress with both similar and unique genes and pathways responding to this stress. Although all three metals are known to be genotoxic, evidence for DNA damage in our study only exists in response to chromium. Nickel induced a hypoxic response as well as inducing genes involved in chromatin structure, perhaps by replacing iron in key proteins. Cadmium distinctly perturbed genes related to endoplasmic reticulum stress and invoked the unfolded protein response leading to apoptosis. With these studies, we have completed the first gene expression comparative analysis of nickel, cadmium, and chromium in H4-II-E-C3 cells.
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| Overall design |
H4-II-E-C3 cells were were exposed to nickel (II) chloride (NiCl2), cadmium chloride (CdCl2), or sodium dichromate (Na2Cr2O7) for 24 hours. The cells were treated with a low, mid, and high concentration of each chemical and shared a control for each biological replicated, and we exposed 4 biologcial replicates for a total of 44 samples.
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| Contributor(s) |
Permenter MG, Lewis JA, Jackson DA |
| Citation(s) |
22110744 |
| Submission date |
Aug 18, 2011 |
| Last update date |
Jul 31, 2017 |
| Contact name |
John A. Lewis |
| E-mail(s) |
john.a.lewis1@us.army.mil
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| URL |
http://usacehr.amedd.army.mil
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| Organization name |
U.S. Army Center for Environmental Health Research
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| Department |
Biomarkers Program
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| Street address |
568 Doughten Drive
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| City |
Ft. Detrick |
| State/province |
MD |
| ZIP/Postal code |
21702-5010 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (44)
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| GSM782283 |
Nickel 140 µM, biological rep1 |
| GSM782284 |
Nickel 400 µM, biologcial rep1 |
| GSM782285 |
Chromium 0.275 µM, biological rep1 |
| GSM782286 |
Chromium 1 µM, biological rep1 |
| GSM782287 |
Chromium 10 µM, biological rep1 |
| GSM782288 |
Cadmium 0.2 µM, biological rep1 |
| GSM782289 |
Cadmium 0.55 µM, biological rep1 |
| GSM782290 |
Cadmium 1.2 µM, biological rep1 |
| GSM782291 |
Control 1, biological rep2 |
| GSM782292 |
Control 2, biological rep2 |
| GSM782293 |
Nickel 40 µM, biological rep2 |
| GSM782294 |
Nickel 140 µM, biological rep2 |
| GSM782295 |
Nickel 400 µM, biologcial rep2 |
| GSM782296 |
Chromium 0.275 µM, biological rep2 |
| GSM782297 |
Chromium 1 µM, biological rep2 |
| GSM782298 |
Chromium 10 µM, biological rep2 |
| GSM782299 |
Cadmium 0.2 µM, biological rep2 |
| GSM782300 |
Cadmium 0.55 µM, biological rep2, repeated microarray |
| GSM782301 |
Cadmium 1.2 µM, biological rep2 |
| GSM782302 |
Control 1, biological rep3 |
| GSM782303 |
Control 2, biological rep3 |
| GSM782304 |
Nickel 40 µM, biological rep3 |
| GSM782305 |
Nickel 140 µM, biological rep3 |
| GSM782306 |
Nickel 400 µM, biologcial rep3 |
| GSM782307 |
Chromium 0.275 µM, biological rep3 |
| GSM782308 |
Chromium 1 µM, biological rep3 |
| GSM782309 |
Chromium 10 µM, biological rep3 |
| GSM782310 |
Cadmium 0.2 µM, biological rep3 |
| GSM782311 |
Cadmium 0.55 µM, biological rep3 |
| GSM782312 |
Cadmium 1.2 µM, biological rep3 |
| GSM782313 |
Control 1, biological rep4 |
| GSM782314 |
Control 2, biological rep4 |
| GSM782315 |
Nickel 40 µM, biological rep4 |
| GSM782316 |
Nickel 140 µM, biological rep4, repeated microarray |
| GSM782317 |
Nickel 400 µM, biologcial rep4, repeated microarray |
| GSM782318 |
Chromium 0.275 µM, biological rep4, repeated microarray |
| GSM782319 |
Chromium 1 µM, biological rep4 |
| GSM782320 |
Chromium 10 µM, biological rep4 |
| GSM782321 |
Cadmium 0.2 µM, biological rep4 |
| GSM782322 |
Cadmium 0.55 µM, biological rep4 |
| GSM782323 |
Cadmium 1.2 µM, biological rep4 |
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| Relations |
| BioProject |
PRJNA145755 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31503_RAW.tar |
114.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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