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Series GSE31564 Query DataSets for GSE31564
Status Public on Aug 25, 2011
Title Gene expression response to bacterial phagocytosis by S2 cells (control and eater RNAi knock down)
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary The Drosophila phagocytic receptor Eater is expressed specifically in phagocytic hemocytes. It contributes to host immune defense and is required for survival of bacterial infections. Eater is involved in recognition and phagocytosis of bacteria.
We used microarrays to determine whether any gene expression changes after bacterial phagocytosis are dependent on the expression of the phagocytic scavenger receptor Eater. We found transcriptional regulation in response to bacterial internalization, but no significant differences between controls and samples in which eater expression had been diminshed by RNAi eater knock down.
 
Overall design Drosophila S2 cells, a hemocyte-derived cell line with phagocytic properties, were exposed to a mixture of Gram-positive and Gram-negative bacteria at conditions of 50% of cell binding. At different time points of synchronized phagocytosis (30, 90 and 180 minutes) total RNA was extracted and subjected to microarray analysis. We compared S2 cells in which Eater expression was decreased by RNA interference with control S2 cells exposed to irrelevant double-stranded (pBR322). Three independent experiments were performed corresponding to three biological replicates. Decreased phagocytosis due to RNAi eater knock down was controlled in all cases.
 
Contributor(s) Kocks C
Citation(s) 22223092
Submission date Aug 22, 2011
Last update date Aug 28, 2018
Contact name Christine Kocks
E-mail(s) kocks@molbio.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Pediatrics/Molecular Biology
Lab Kocks
Street address 185 Cambridge St CPZN7.250
City Boston
State/province Massachusetts
ZIP/Postal code 02446
Country USA
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (24)
GSM783334 S2 RNAi-control no bacteria 30min rep1
GSM783335 S2 RNAi-control no bacteria 30min rep2
GSM783336 S2 RNAi-control no bacteria 30min rep3
Relations
BioProject PRJNA145693

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31564_RAW.tar 51.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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