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Series GSE31695 Query DataSets for GSE31695
Status Public on Aug 01, 2012
Title Comparison of Differences in Gene Expression in the Ventral Tegmental Area of 5 Pairs of Rat Lines Selectively Bred for High or Low Alcohol Consumption
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The objective of this study was to determine common innate differences in gene expression in the ventral tegmental area (VTA) among the selectively bred (a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats: (b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (both replicates); (c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats; and (d) Sardinian alcohol-preferring (sP) vs. alcohol-nonpreferring (sNP) rats. There were between 350 and 1400 unique named genes that were significantly different between the individual line-pairs. Gene Ontology (GO) and Ingenuity Pathways analyses indicated significant categories and networks in common for up to 3 line-pairs, but not for all 5 line-pairs; there were few genes in common between any of the line-pairs in these categories and networks. The overall ANOVAs of the combined data for the 5 line-pairs indicated over 1300 significant differences in expression of named genes. Ingenuity analysis revealed (a) several significant networks with clusters of genes associated with App, Egfr, Ccnd1, Itga2b, Rxra and Vcl; and (b) changes in genes within networks associated with dopamine, the glutamate synapse, Nfkb signaling, IL pathways and integrin. There were 22 genes that were significantly different in the overall ANOVA and were significantly different (in the direction) in at least 3 line-pairs, e.g., Crebl2, Gsta4, Itga9 & Itg2. In conclusion, the findings suggest that (a) different innate mechanisms may be contributing to vulnerability to high alcohol drinking behavior among the selectively bred lines, and (b) small contributions in expression of multiple genes within certain transmitter systems and intracellular signaling pathways may contribute to the disparate alcohol drinking characteristics of the 5 line-pairs.
 
Overall design Comparison of Differences in Gene Expression in the Ventral Tegmental Area of 5 Pairs of Rat Lines Selectively Bred for High or Low Alcohol Consumption.
 
Contributor(s) McBride WJ, Kimpel MW, McClintick JN, Ding Z, Bell RL, Hyytia P, Colomobo G, Edenberg HJ
Citation(s) 22579914
Submission date Aug 26, 2011
Last update date Jul 31, 2017
Contact name Mark William Kimpel
E-mail(s) mkimpel@iupui.edu
Organization name Indiana University
Department Psychiatry
Lab W.J. McBride
Street address 15032 Hunter Court
City Westfield
State/province IN
ZIP/Postal code 46074
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (91)
GSM786646 Line Pair HAD2.LAD2 - Alc Pref Pr - animal H2.2 [ventral tegmental area]
GSM786647 Line Pair HAD2.LAD2 - Alc Pref Pr - animal H2.3 [ventral tegmental area]
GSM786648 Line Pair HAD2.LAD2 - Alc Pref NPr - animal L2.11 [ventral tegmental area]
This SubSeries is part of SuperSeries:
GSE31709 Comparison of Differences in Gene Expression in brain of 5 Pairs of Rat Lines Selectively Bred for High or Low Alcohol Consumption
Relations
BioProject PRJNA155009

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31695_RAW.tar 246.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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