GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Series GSE31729

Query DataSets for GSE31729

Status

Public on Aug 30, 2011

Title

Lack of effect in desensitization with intravenous immunoglobulin and rituximab in highly-sensitized patients

Organism

Experiment type

Expression profiling by array

Summary

Background: We aimed to investigate the effects of intravenous immune globulin (IVIG) and rituximab desensitization treatment on kidney transplant rate and blood gene expression profiles by microrarrays. Methods: We enrolled patients with PRA levels >50% and on the deceased-donor waiting list for >5 years. Patients received IVIG (2.0 g/kg) on day 0 and 30; and rituximab (375 mg/m2) on day 15. The antibodies with mean fluorescence intensity (MFI) values > 5,000 were reported to UNET as unacceptable antigens. The gene expression profiles of blood samples collected in PAXGene tube were studied by Affymetrix HuGene 1.0 ST expression arrays. Results: 40 of the 415 patients (10%) on the waiting list were eligible for desensitization treatment and 11 completed the treatment. While 15 of the remaining 29 patients (52%) received a transplant without therapy, only 2 of the 11 desensitized patients (18%) received transplant during a median follow-up of 217 days. While there were no statistically significant difference in demographics, desensitized patients had higher cPRA values (97% vs. 77%, p=0.0005) and more number of unacceptable antigens (39 vs. 10, p=0.0001). There was no significant change in the mean number of unacceptable antigens (39 ± 22 versus 39 ± 23) or reduction in the mean MFI values (11,333 ± 3,133 vs 11,289 ± 3,386). Analysis of genes chosen as significantly differentially expressed revealed downregulation of genes involved in B cells and immune system (CD79a, B and T lymphocyte associated transcript, B cell scaffold protein, CD22, CXCR5, fas apoptotic inhibitory protein). Gene set enrichment analysis using Pathogenesis Based Transcripts created by Edmonton Group demonstrated significant downregulation of B cell associated (p=0.04) and immunoglobulin transcripts (p=0.03). Conclusion: Although, desensitization with IVIG and rituximab decreases the expression of B cell and immunoglobulin associated transcripts, it was not successful in increasing kidney transplant rate or in decreasing the number of unacceptable antigens.

Overall design

Total of 28 arrays included in this study, which corresponding to 9 individuals with paired pre/post treatment samples and an additional 10 untreated control individuals.
pair_analysis_normData.txt for paired analysis of pre/post treatment;
ordinary_analysis_normData.txt for non-paired analysis include all samples, except for 38 Pax V0 and 56 Pax V1, which shows technicial bias and lowest array quality, hence removed from analysis.

Contributor(s)

Akalin E, Bao Y, Calder RB, Marfo K, Ling M, Ye B, Greenstein S, Chapochnick-Friedman J, Glicklich D, de Boccardo G, Kinkhabwala M

Citation missing

Has this study been published? Please login to update or notify GEO.

Submission date

Aug 29, 2011

Last update date

Jun 07, 2023

Contact name

Bin Ye

E-mail(s)

bin.ye@einstein.yu.edu

Phone

718-678-1147

Organization name

Albert Einstein College of Medicine

Department

Genetics

Street address

1301 Morris Park Ave

City

New York

State/province

NY

ZIP/Postal code

10461

Country

USA

Platforms (1)

[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]

Samples (44)

More...

GSM787652	patient 38 after treatment (non-paired analysis)
GSM787653	patient 40 before treatment (non-paired analysis)
GSM787654	patient 40 after treatment (non-paired analysis)

GSM787655	patient 56 before treatment (non-paired analysis)
GSM787656	patient 59 before treatment (non-paired analysis)
GSM787657	patient 59 after treatment (non-paired analysis)
GSM787658	patient 61 before treatment (non-paired analysis)
GSM787659	patient 61 after treatment (non-paired analysis)
GSM787660	patient 62 before treatment (non-paired analysis)
GSM787661	patient 62 after treatment (non-paired analysis)
GSM787662	patient 69 before treatment (non-paired analysis)
GSM787663	patient 69 after treatment (non-paired analysis)
GSM787664	patient 108 before treatment (non-paired analysis)
GSM787665	patient 108 after treatment (non-paired analysis)
GSM787666	patient 112 before treatment (non-paired analysis)
GSM787667	patient 112 after treatment (non-paired analysis)
GSM787668	patient 6 untreated control (non-paired analysis)
GSM787669	paitent 18 untreated control (non-paired analysis)
GSM787670	patient 26 untreated control (non-paired analysis)
GSM787671	patient 31 untreated control (non-paired analysis)
GSM787672	paitent 32 untreated control (non-paired analysis)
GSM787673	patient 55 untreated control (non-paired analysis)
GSM787674	patient 91 untreated control (non-paired analysis)
GSM787675	paitent 134 untreated control (non-paired analysis)
GSM787676	patient 135 untreated control (non-paired analysis)
GSM787677	patient 139 untreated control (non-paired analysis)
GSM787678	patient 38 before treatment (paired analysis)
GSM787679	patient 38 after treatment (paired analysis)
GSM787680	patient 40 before treatment (paired analysis)
GSM787681	patient 40 after treatment (paired analysis)
GSM787682	patient 56 before treatment (paired analysis)
GSM787683	patient 56 after treatment (paired analysis)
GSM787684	patient 59 before treatment (paired analysis)
GSM787685	patient 59 after treatment (paired analysis)
GSM787686	patient 61 before treatment (paired analysis)
GSM787687	patient 61 after treatment (paired analysis)
GSM787688	patient 62 before treatment (paired analysis)
GSM787689	patient 62 after treatment (paired analysis)
GSM787690	patient 69 before treatment (paired analysis)
GSM787691	patient 69 after treatment (paired analysis)
GSM787692	patient 108 before treatment (paired analysis)
GSM787693	patient 108 after treatment (paired analysis)
GSM787694	patient 112 before treatment (paired analysis)
GSM787695	patient 112 after treatment (paired analysis)

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE31729_RAW.tar	179.8 Mb	(http)(custom)	TAR (of CEL)
GSE31729_ordinary_analysis_normData.txt.gz	4.0 Mb	(ftp)(http)	TXT
GSE31729_pair_analysis_normData.txt.gz	4.0 Mb	(ftp)(http)	TXT
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |